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A Method for Early Sex Determination of Oval Pomfret

A technology of oval pomfret and sex, applied in fish farming, application, climate change adaptation and other directions, can solve the difficulty of inducing labor and establishment of breeding families, unfavorable development of oval pomfret aquaculture industry, and technical requirements for inspectors Advanced problems, to achieve the effect of easy determination method, obvious differences in gonad characteristics, and improved observation efficiency

Active Publication Date: 2016-04-06
GUANGXI ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ultrasonic method is used to study the gonads of juvenile fish. Juvenile fish need special treatment, and the ultrasonic detector is expensive, and the technical requirements of the inspectors are high, which is not suitable for the needs of the majority of farmers.
The film-making method to study the gonads of juvenile fish requires a variety of relatively complex chemical reagents, many operating steps, strict technical requirements, and a biological microscope, which is not suitable for the identification of a large number of samples
[0004] At present, there are no literature reports and invention patents on the sex identification method of ovata pomfret, which has caused great difficulties in the induction of spawning and the establishment of breeding families in the breeding of ovata pomfret, which is not conducive to the breeding of ovata pomfret industry development

Method used

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  • A Method for Early Sex Determination of Oval Pomfret

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Take the first-year-old oval pomfret, cut off the left body wall to expose the internal organs.

[0027] 2. Find the intestinal tract and the left and right gonads from the cloaca toward the abdominal cavity. The gonads are located on both sides of the back of the swim bladder. The gonads are long and thin, milky white, and small in size.

[0028] 3. Carefully peel off other visceral tissues other than the gonads, take out the complete unilateral gonads, cut them transversely from the middle, observe with the naked eye and under an anatomical microscope, they are all solid structures.

[0029] 4. Determine that the gonad is the testis, and the oval pomfret is male.

[0030] To verify the accuracy of this method, the following slice experiments were performed:

[0031] The gonads on the other side were taken out and fixed in 4% PFA solution for 24 hours. After dehydration with 70%-100% gradient ethanol, routine paraffin sections and HE staining were performed. The m...

Embodiment 2

[0034] 1. Take the first-year-old oval pomfret, cut off the left body wall to expose the internal organs.

[0035] 2. Find the intestinal tract and the left and right gonads from the cloaca towards the abdominal cavity. Observe in situ that the gonads are located on both sides of the back of the swim bladder, close to the body wall and kidneys.

[0036] 3. Carefully peel off other visceral tissues other than the gonads, take out the complete unilateral gonads, cut them transversely from the middle, observe with the naked eye and under an anatomical microscope, they are all hollow structures, and granular oocytes attached to the inner wall of the egg cavity can be seen under the microscope cell.

[0037] 4. It is determined that the gonad is an ovary, and the oval pomfret is a female.

[0038] To verify the accuracy of this method, the following slice experiments were performed:

[0039] The gonads on the other side were taken out and fixed in 4% PFA solution for 24 hours. Af...

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Abstract

The invention discloses a method for judging the early sex of the oval pomfret. The young oval pomfret is taken, the left body wall is cut off, the gonad is found, the characteristics of the gonad are observed in situ, the gonad is taken out and dissected, and the anatomical surface of the gonad is observed. The sex of Pompano ovata was determined by analyzing the gonad characteristics. The method is simple in equipment, fast in operation, accurate in results, and does not use toxic chemical reagents. It is a convenient and efficient method for early sex determination of pomfret ovata, and can provide reliable materials for further research on sex-specific molecular markers in pomfret ovata. .

Description

technical field [0001] The invention belongs to fish sex identification technology in the technical field of aquatic organisms, and more specifically, the invention relates to a method for early sex identification of ovate pomfret. Background technique [0002] Oval Pomfret ( Trachinotus ovatus ), commonly known as golden pomfret, yellow waxed pomfret, belongs to Perciformes, Trevalidae. The egg-shaped pomfret has tender meat, delicious taste and high nutritional value. It is a valuable edible fish and is very popular in the market. At the same time, the oval pomfret has fast growth rate, strong disease resistance, high yield and good profit, and is a very popular breeding species for farmers. However, it takes a long time for the ovoid pomfret to reach sexual maturity, which generally takes 4 to 5 years, and during the entire life of the ovate pomfret, both male and female fish do not have gender-specific appearance characteristics. Therefore, it is difficult for breeder...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 彭金霞李咏梅陈晓汉彭敏蒋小珍韦嫔媛蒋伟明黎铭
Owner GUANGXI ACADEMY OF FISHERY SCI
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