The invention discloses a multiple rapid detection method, a detection primer group and a kit for three food-borne pathogenic bacteria. According to the invention, upstream and downstream primers of an external primer and upstream and downstream primers of an internal primer of a rapid detection primer group of salmonella correspondingly have sequences as shown in SEQNo.1, SEQNo.2, SEQNo.3 and SEQNo.4; upstream and downstream primers of an external primer and upstream and downstream primers of an internal primer of a rapid detection primer group of staphylococcus aureus have sequences as shown in SEQNo.5, SEQNo.6, SEQNo.7 and SEQNo.8; upstream and downstream primers of an external primer and upstream and downstream primers of an internal primer of a rapid detection primer group of Listeria monocytogenes have sequences as shown in SEQNo.9, SEQNo.10, SEQNo.11 and SEQNo.12. By using the method in the invention, the defect that the prior art can not be used for simultaneously judging whether multiple pathogenic bacteria exist in the same system at the same time is overcome, and whether the same detected sample contains one or more of salmonella, staphylococcus aureus and Listeria monocytogenes can be correctly detected.