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A method for manufacturing artificial soft tissue body with vascular network flow channel

A manufacturing method and soft tissue technology, applied in the field of artificial soft tissue preparation, can solve the problems of small repair volume, stent vascularization, insufficient oxygen and nutrient supply for cell growth, etc., and achieve the promotion of vascularization, a wide range of selectivity, and strong applicability. Effect

Active Publication Date: 2015-08-26
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for manufacturing an artificial soft tissue body with a vascular network flow channel to solve the problems of small repair volume, vascularization of the scaffold, and oxygen and nutrient supply for cell growth in the prior art in the manufacture of hydrogel scaffolds. Insufficient problems such as

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  • A method for manufacturing artificial soft tissue body with vascular network flow channel

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Effect test

Embodiment 1

[0028] 1) According to the structure of the arterial vascular network of the organism and the diffusion effect of oxygen and nutrients, use CAD software to design a soft tissue stent model with vascular structure, and layer the designed soft tissue stent model one by one equidistantly, and obtain each layer after layering support, and then respectively manufacture each photomask plate used to manufacture each layer of support;

[0029] The diffusion distance of oxygen and nutrients in the designed soft tissue scaffold model with vascular structure is 200 μm. Considering the hydrogel forming process, the designed blood vessel diameter is 800 μm; the layered thickness of the model is related to the hydrogel forming process and the vascular pore size , the layered thickness of the soft tissue scaffold model is designed to be 800 μm;

[0030] Each photomask is designed and processed according to the structure of each layer of brackets. The specific manufacturing method is: first k...

Embodiment 2

[0041] 1) According to the structure of the arterial vascular network of the organism and the diffusion effect of oxygen and nutrients, use CAD software to design a soft tissue stent model with vascular structure, and layer the designed soft tissue stent model one by one equidistantly, and obtain each layer after layering support, and then respectively manufacture each photomask plate used to manufacture each layer of support;

[0042] The diffusion distance of oxygen and nutrients in the designed soft tissue scaffold model with vascular structure is 200 μm. Considering the hydrogel forming process, the designed blood vessel diameter is 1 mm; the layered thickness of the model is related to the hydrogel forming process and the vascular pore size , the layered thickness of the soft tissue scaffold model is designed to be 1mm;

[0043] Each photomask is designed and processed according to the structure of each layer of brackets. The specific manufacturing method is: first keep t...

Embodiment 3

[0055] 1) According to the structure of the arterial vascular network of the organism and the diffusion effect of oxygen and nutrients, use CAD software to design a soft tissue stent model with vascular structure, and layer the designed soft tissue stent model one by one equidistantly, and obtain each layer after layering support, and then respectively manufacture each photomask plate used to manufacture each layer of support;

[0056] The diffusion distance of oxygen and nutrients in the designed soft tissue scaffold model with vascular structure is 200 μm. Considering the hydrogel forming process, the designed vessel diameter is 900 μm; the layered thickness of the model is related to the hydrogel forming process and the vascular pore size , the layered thickness of the soft tissue scaffold model is designed to be 900 μm;

[0057] Each photomask is designed and processed according to the structure of each layer of brackets. The specific manufacturing method is: first keep th...

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Abstract

The invention provides a method for manufacturing an artificial soft tissue body carried with a vascular net flow channel. The method comprises the following steps of: firstly designing a soft tissue scaffold model with a vascular structure, layering the soft tissue scaffold model one by one at equal distance, and manufacturing photomask plates of various layers; then uniformly mixing a cell and a collagen solution, and injecting a photocuring hydrogel and a photoinitiator to obtain a photocuring composite solution; injecting the photocuring composite solution to a work table, covering with the photomask plates, curing the photocuring composite solution by adopting a surface exposure technology, and then carrying out curing accumulation layer by layer to obtain a photocured hydrogel soft tissue scaffold with the vascular structure; planting vascular endothelial cells to the vascular structure of the photocured hydrogel soft tissue scaffold, wherein the vascular endothelial cells are attached to the surface of a vascular channel; carrying out static culture and dynamic culture in vitro to obtain the artificial soft tissue body carried with the vascular net flow channel. The method provided by the invention can solve the problems of survival of cells inside a large tissue engineering soft tissue scaffold, and soft tissue scaffold vessel network manufacturing and vascularization in the large injury repair of a soft tissue.

Description

technical field [0001] The invention relates to the field of artificial soft tissue preparation, and specifically relates to a method for manufacturing an artificial soft tissue with vascular network channels. Background technique [0002] Soft tissue plays a very important role in the body, and heredity, trauma, disease and aging often cause the loss or loss of function of soft tissue, which seriously threatens human health. Therefore, the regeneration of functional soft tissue has always been an important issue in the field of regenerative medicine. The development of tissue engineering has brought new hope for the treatment of soft tissue damage and loss of function. It applies engineering and biological principles, combined or alone with biomaterials, cells and growth factors, to research and develop the ability to maintain, repair or even replace damaged tissues. Tissue engineered substitutes for damaged soft tissue. [0003] Facing the skin tissue engineering scaffol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071A61L27/38A61L27/44A61L27/52
Inventor 连芩陈成李涤尘庄佩贺健康刘亚雄靳忠民
Owner XI AN JIAOTONG UNIV
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