Capillary electrophoresis analysis system utilizing micro-injection pump to drive liquid flows

Abstract

The invention discloses a capillary electrophoresis analysis system utilizing a micro-injection pump to drive liquid flows. The capillary electrophoresis analysis system comprises a direct-current high-voltage power supply, a nanoliter sample-injection valve with a built-in nanoliter sample ring, the micro-injection pump, a capillary three-way valve, a separation capillary, a detector and an electrophoresis waste liquid bottle with an electrophoresis buffer solution and a bottle plug. The capillary electrophoresis analysis system utilizes the micro-injection pump to drive liquid flows at a flowing rate of nL/min so that the liquid flows can integrally move in the capillary from a positive pole to a negative pole, and the liquid flows driven by the micro-injection pump replace electroosmotic flows so that all ingredients of the sample are separated because of different electrophoresis speeds. The capillary electrophoresis analysis system has the characteristics of high separation efficiency, fast analysis speed, simple processes and good reappearance.

Description

(1) Technical field [0001] The invention relates to a capillary electrophoresis analysis system, in particular to a capillary electrophoresis analysis system driven by a micro-injection pump and an operating method thereof. (2) Background technology [0002] Since the 1970s, capillary electrophoresis has been widely used in chemistry, biology, environmental science, food, pharmacy, medicine and other fields due to its advantages of high separation efficiency, low reagent consumption, and short analysis time. Zone capillary electrophoresis is the most commonly used separation method in capillary electrophoresis. It has good separation ability for ionic compounds and has been widely used in small molecular weight such as amino acids, drugs, metal ions, and toxic and harmful substances in environmental samples. The separation and analysis of compounds can also be used for the separation of biological macromolecules such as proteins. [0003] The quartz capillary column used in...

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Problems solved by technology

[0004] Since the temperature, pH, composition and concentration of the electrolyte solution, the activation state of the inner wall of the capillary and the adsorption of the solute all affect the size of the electroosmotic flow, and there are many factors affecting the injection volume of the capillary electrophoresis, the separation and determination of the capillary electrophoresis are important. The reproducibility is not as good as that of high performance liquid chromatography and gas chromatography, which is limited in practical application

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