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Method for preparing mogroside V from siraitia grosvenori

A technique for mogroside and mogroside, which is applied in the field of preparing mogroside V, can solve the problems of high production cost, deepened color of the extract, high energy consumption, etc., and achieves the effects of avoiding damage, short time and high yield

Active Publication Date: 2014-10-08
GUILIN SHILI SCI & TECH COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are certain defects in the Luo Han Guo extract obtained by the above method: the obtained extract has a deep color and has a strong taste of traditional Chinese medicine and a strong after-bitter taste, the content of active ingredients is low, and the color of the extract is deepened, which is not conducive to the follow-up. Disadvantages of decolorization and purification treatment, large energy consumption and high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for preparing mogroside V from Luo Han Guo, specifically comprising the steps of:

[0020] 1) Raw material processing: take 100kg of fresh Luo Han Guo, wash and crush;

[0021] 2) High-pressure treatment: Put the crushed Luo Han Guo into a high-pressure tank, add 100kg of pure water, and pressurize to 4kgf / cm 2 , make the pressure uniform, and keep it for 15 minutes, return to normal pressure, and get about 200kg of paste slurry;

[0022] 3) Supercritical CO 2 Fluid extraction: put the high-pressure treated Luo Han Guo paste slurry in the extraction kettle, and use supercritical CO 2 The fluid is the extraction solvent, and the ethanol solution with a volume concentration of 60% is used as the entrainer for supercritical extraction. The mass ratio of the paste slurry to the extraction kettle is 1:4, and the volume ratio of the ethanol solution to the extraction kettle is 1. : 2; the extraction temperature is 60°C, the extraction pressure is 15Mpa, and the ex...

Embodiment 2

[0025] A method for preparing mogroside V from Luo Han Guo, specifically comprising the steps of:

[0026] 1) Raw material processing: take 100kg of fresh Luo Han Guo, wash and crush;

[0027] 2) High-pressure treatment: Put the crushed Luo Han Guo into a high-pressure tank, add 100kg of pure water, and pressurize to 4kgf / cm 2 , make the pressure uniform, and keep it for 10 minutes, return to normal pressure, and get about 200kg of paste slurry;

[0028] 3) Supercritical CO 2 Fluid extraction: put the high-pressure treated Luo Han Guo paste slurry in the extraction kettle, and use supercritical CO 2 The fluid is the extraction solvent, and the ethanol solution with a volume concentration of 50% is used as the entrainer for supercritical extraction. The mass ratio of the paste slurry to the extraction tank is 1:4, and the volume ratio of the ethanol solution to the extraction tank is 1. : 2; the extraction temperature is 50°C, the extraction pressure is 20Mpa, and the extrac...

Embodiment 3

[0031] A method for preparing mogroside V from Luo Han Guo, specifically comprising the steps of:

[0032] 1) Raw material processing: take 100kg of fresh Luo Han Guo, wash and crush;

[0033] 2) High-pressure treatment: Put the crushed Luo Han Guo into a high-pressure tank, add 100kg of pure water, and pressurize to 4kgf / cm 2 , make the pressure uniform, and keep it for 12 minutes, return to normal pressure, and get about 200kg of paste slurry;

[0034] 3) Supercritical CO 2 Fluid extraction: put the high-pressure treated Luo Han Guo paste slurry in the extraction kettle, and use supercritical CO 2 The fluid is the extraction solvent, and the ethanol solution with a volume concentration of 55% is used as the entrainer for supercritical extraction. The mass ratio of the paste slurry to the extraction kettle is 1:4, and the volume ratio of the ethanol solution to the extraction kettle is 1. : 2; the extraction temperature is 55°C, the extraction pressure is 20Mpa, and the ex...

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Abstract

The invention discloses a method for preparing mogroside V from siraitia grosvenori. The method comprises the following steps of carrying out raw material treatment, high pressure treatment and supercritical CO2 fluid extraction, and collecting an extracting solution obtained after extracting; centrifuging, and carrying out HP-20ss resin column chromatography on supernatant; then, eluting by using a 50-60% ethanol solution, concentrating, and carrying out spray drying to obtain mogroside V. The method has the advantages of high content, simple process, low production cost and the like, and is suitable for production.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for preparing mogroside V from Luo Han Guo. Background technique [0002] Mangosteen, known as the "fairy fruit", is a precious and precious Cucurbitaceae plant unique to my country, and is known as a good medicine and good fruit. Luo Han Guo belongs to the Cucurbitaceae plant. It is a multi-purpose medicinal and health-care fruit. One of the traditional Chinese medicines for food. According to the records in "Dictionary of Chinese Medicine" and other books, Luo Han Guo is extremely sweet, cool in nature, non-toxic, and enters the spleen and lungs. , invigorating the spleen, calming, quenching thirst, promoting gastrointestinal function, inhibiting asthma and lowering blood pressure, etc. It is often used for lung fire dry cough, sore throat, aphonia, intestinal dryness and constipation. [0003] The main component of Luo Han Guo extract is mogrosid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCY02P20/54
Inventor 何伟平
Owner GUILIN SHILI SCI & TECH COMPANY
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