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Bacillus thuringiensis and its solid medium preparation method

A technology of Bacillus thuringiensis and solid culture medium, which is applied in the field of microorganism and organic fertilizer manufacturing and microbial culture medium, can solve problems such as fertility decline and soil organic matter content reduction, and achieve the effects of ensuring activity, low production cost and convenient material extraction.

Active Publication Date: 2017-01-11
沈阳壅源生物有机肥有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since my country's agricultural production has long relied on chemical fertilizers and applied them in large quantities, the content of organic matter in the soil has decreased and its fertility has declined.

Method used

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  • Bacillus thuringiensis and its solid medium preparation method
  • Bacillus thuringiensis and its solid medium preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0026] 1. Select 400kg of fresh broiler manure with a water content of 73.5%, add 1200kg of water to dilute, stir well, remove the larger feathers and sundries, and use layering to remove the bottom sand.

[0027] 2. Dilute the dung water in the first step, and use a press with a 50-mesh filter screen to squeeze out excess water to obtain 230kg of material with a water content of 63.7%, add 40kg of wheat bran, and fully stir to make the mixed material into a loose state. Put into a drum drier, dry at 110° C. for 30 minutes, and obtain 145 kg of chicken manure treatment raw materials with a water content of 29.7%.

[0028] 3. Take 500mL of water and heat to dissolve 200g of bone marrow extract, then add 150mL of bamboo vinegar, add 650g of superphosphate to the 145kg of chicken manure treatment raw materials obtained in step 2, mix well, and sterilize at 115°C for 35min.

[0029] 4. Add 200 g of the cultured Bacillus thuringiensis powder, mix well, place it at 35°C and cultivat...

Embodiment 2

[0031] 1. Select 100kg of fresh broiler manure with a water content of 68.3%, add 400kg of water to dilute, stir well, remove the larger feathers and sundries, and use layering to remove the bottom sand.

[0032] 2. Dilute the dung water in the first step, and use a 60-mesh filter to squeeze out excess water to obtain 74kg of material with a water content of 66.4%, add 8kg of wheat bran, stir well to make the mixed material loose, and send it to the drum dryer , dried at 110° C. for 30 minutes to obtain 30 kg of chicken manure treatment raw materials with a water content of 28.4%.

[0033] 3. Take 200mL of water and heat to dissolve 20g of bone marrow extract, then add 30mL of bamboo vinegar, add 120g of superphosphate to the 30kg of chicken manure treatment raw materials obtained in step 2, mix well, and sterilize at 115°C for 35min.

[0034] 4. Add 50 g of the cultured Bacillus thuringiensis powder, mix well, place it at 35°C and cultivate it for 48 hours, and turn it twice ...

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Abstract

The invention provides a preparation method for a Bacillus thuringiensis solid medium, a method for culturing a Bacillus thuringiensis inoculant by using the medium and application of cultured Bacillus thuringiensis in production of organic fertilizers, belonging to the technical field of microbial cultivation and application. The preparation method for the solid medium comprises the following steps: S1, preparing a treated chicken manure raw material; and S2, weighing 10 kg of the treated chicken manure raw material obtained in the S1, adding 5 to 20 g of marrow extract, 30 to 50 g of calcium superphosphate and 10 to 15 ml of a bamboo vinegar liquid, carrying out full mixing and then carrying out sterilization at a temperature of 115 DEG C for 30 to 35 min so as to prepare the Bacillus thuringiensis solid medium. A microbial agent of the Bacillus thuringiensis cultured by using the medium is applicable to fermentation production of aerobiotic organic fertilizers and is required to be used when temperature drops to less than 45 DEG C after high temperature fermentation. According to the invention, through addition of a beneficial microbial flora in a fertilizer, the organic fertilizer is endowed with a new function, and dual effects of a biological fertilizer and a biological pesticide are realized.

Description

technical field [0001] The invention relates to the technical field of microbial culture medium, microorganism and organic fertilizer production, in particular to a method for preparing a solid medium for Bacillus thuringiensis, a method for preparing Bacillus thuringiensis using the medium, and the application of Bacillus thuringiensis in the production of organic fertilizers. Background technique [0002] Fertilizer is the "grain" of crop production, and fertilization is the most common and direct measure to increase agricultural production. As a traditional agricultural country, my country has a good tradition of using organic fertilizers in agricultural production. After entering the 1980s, chemical fertilizers began to be used in large quantities in our country, making great contributions to our country's agricultural production. Considering the basic national conditions of my country's population of 1.3 billion, 23% of the world's population can be fed with 9% of the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/00C12R1/07
CPCY02W30/40
Inventor 陶思源董生忠王鹏飞丁霖王梦圆
Owner 沈阳壅源生物有机肥有限公司
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