Molecular marker, primer and application thereof closely linked to maize bacterial wilt resistance gene

Abstract

The invention discloses a molecular marker which is closely linked with corn bacterial wilt resistance genes. The molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 in a sequence table or a nucleotide sequence as shown in SEQ ID NO.2 in the sequence table. A primer pair for amplifying the molecular marker is a nucleotide sequence as shown in SEQ ID NO.3 in the sequence table and a nucleotide sequence as shown in SEQ ID NO.4 in the sequence table. When the molecular marker is applied to corn bacterial wilt detection, amplification is performed through PCR, if an obtained PCR amplification product is a 234bp basic group fragment, the corn to be detected is of a bacterial wilt resistant variety, and if the obtained PCR amplification product is a 274bp alkali group fragment, the corn to be detected is of a bacterial wilt infected variety. By adopting the method, whether the corn has the bacterial wilt resistance or not can be detected before the corn is seeded, selective seeding can be achieved, the breeding time is shortened, and the method is rapid, simple and convenient and can be widely applied to assisted breeding of corn.

Description

Technical field

[0001] The invention belongs to the field of agricultural biotechnology, and specifically relates to a method for detecting corn bacterial wilt resistance, PCR primers and applications thereof. Background technique

[0002] Conventional breeding methods based on phenotypic selection have shortcomings such as low selection efficiency and long breeding cycles. It is urgent to inject modern molecular techniques, supplemented by high-efficiency genotype-directed selection, to quickly and efficiently breed excellent new maize varieties. With the rapid development of molecular biology and genomics, molecular marker technology has become more widely used. PCR-based molecular markers such as microsatellites or SSR (simplesequence repeat) have the characteristics of high polymorphism rate, relative stability, simple and rapid detection methods and easy operation, and are widely used. Since molecular marker-assisted selection is not susceptible to environmental factors and...

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