Apparatus for rapidly detecting cow's milk allergens in food, and making method thereof

A milk allergy and food technology, applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems of low contrast between band and background, difficulty in achieving sensitivity, detection stability, and difficulty in multi-inspection and connection , to achieve fast and accurate qualitative and quantitative detection, to achieve multi-inspection and joint inspection, and to achieve the effect of long service life

Inactive Publication Date: 2014-10-15
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] (1) The colloidal gold labeling process is an electrostatic adsorption process, which is a physical adsorption, so it has poor stability in the liquid phase.
[0008] Often cause the labeled protein molecules to fall off again
[0009] (2) The result is judged by displaying a single purple-red strip, which is single in color, and it is difficult to achieve multiple detection and linkage.
[0012] The bands have little contrast to the background, limiti

Method used

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  • Apparatus for rapidly detecting cow's milk allergens in food, and making method thereof
  • Apparatus for rapidly detecting cow's milk allergens in food, and making method thereof
  • Apparatus for rapidly detecting cow's milk allergens in food, and making method thereof

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Embodiment 1

[0053] 1. Assembly of fluorescent labeling device;

[0054] 1. Preparation of NC membrane:

[0055] Preparation of detection area and quality control area: adjust the concentration of milk allergen to 1.0 mg / mL with 0.05 M PBS pH 7.2 (containing 5% sucrose and 0.05% Tween-20), and spray the resulting solution on the membrane for detection Area, adjust the concentration of goat anti-rabbit IgG to 1.0 mg / mL with 0.01 M PBS pH 7.2 (containing 5% sucrose and 0.05% Tween-20), and spray the resulting solution on the quality control area on the membrane. The amount of sprayed film was 0.8 μL / cm, the distance between the two areas was 5 mm, and the quality control area was 2 mm from one end of the NC film. After drying at 37 °C overnight, it was stored in a dry environment for later use.

[0056] 2. Preparation of fluorescent microsphere pads:

[0057] Preparation of fluorescent microsphere-labeled bovine milk allergen polyclonal antibody: take 1 mg of commercially available fluores...

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Abstract

The invention discloses an apparatus for rapidly, conveniently, efficiently and quantitatively detecting cow's milk allergens in a food, and a making method thereof, and belongs to a fluorescence microsphere labeled detection technology. The apparatus comprises a special property labeled biological macromolecule with biological specificity and a polymeric material film with adsorption mobility as carriers, and detection is carried out in the portable and small square apparatus. The special property of a tag is used to quantitatively detect a trace amount of cow's milk allergens contained in the matrix of the food. The special tag overcomes the problems of instability and low detection value of common tags. The apparatus is mainly used in the quantitative detection of residual cow's milk allergens in food safety.

Description

technical field [0001] The invention belongs to the field of detection of milk allergens in food in food safety, and in particular relates to a fluorescent microsphere labeling device for qualitatively and quantitatively rapidly detecting milk allergens in food and a preparation method thereof. Background technique [0002] In recent years, with the improvement of people's living standards, food safety issues have become more and more prominent, including food allergies. Food allergy refers to an allergic reaction of the body to a certain substance or component in food. It is a hypersensitivity reaction mediated by IgE and non-IgE, which can cause reactions in the skin, gastrointestinal tract, and respiratory system. In severe cases, it may even lead to systemic allergic reactions or shock. [0003] Cow milk product is a kind of nutrient-rich protein product, and it is also one of the eight common allergens. Its sensitization has seriously affected the health of infants and...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/533G01N21/64
CPCG01N33/558G01N21/6402G01N33/533
Inventor 赖卫华倪小琴刘道峰山珊彭涛
Owner NANCHANG UNIV
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