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Recombinant escherichia coli strains

A technology for recombining Escherichia coli and Escherichia coli, which is applied in the direction of bacteria, cationic antimicrobial peptides, and medical raw materials derived from bacteria, etc. It can solve the problems of inactivity of fusion products, increased production of soluble products, and insolubility

Inactive Publication Date: 2014-10-29
医药中心有限公司
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AI Technical Summary

Problems solved by technology

However, this raises another problem: most fusion products are inactive or produced in an insoluble form
A fusion expression system of thioredoxin (TrxA) was reported to result in increased yield of soluble product

Method used

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  • Recombinant escherichia coli strains
  • Recombinant escherichia coli strains
  • Recombinant escherichia coli strains

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Embodiment Construction

[0011] According to a first aspect, the present invention relates to recombinant Escherichia coli (E. coli) Nissle 1917 cells transformed with a nucleic acid encoding a defensin protein or a derivative thereof.

[0012] In the context of the present invention, the term "nucleic acid sequence" refers to a heteropolymer of nucleotides or a sequence of these nucleotides. The term "nucleic acid" includes RNA as well as DNA, including cDNA, genomic DNA, and synthetic (eg, chemically synthesized) nucleic acids, and the like.

[0013] As used herein, the term "derivative" refers to a nucleic acid derivative encoding a defensin protein, which comprises one or more substitutions, insertions and / or deletions compared to the original defensin nucleic acid sequence. Preferably, these derivatives have 1, 2, 3, 4 or more nucleotides deleted at the 5'-end or 3'-end or within the nucleic acid sequence, or these nucleotides are replaced by other core Nucleotide replacement.

[0014] The term...

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Abstract

The present invention is directed to a recombinant E. coli Nissle 1917 (EcN) cell transformed with a nucleic acid coding for a defensin protein or a derivative thereof. The invention is further directed to a pharmaceutical composition comprising this cell and a pharmaceutically acceptable carrier as well as a method of producing a recombinant E. coli Nissle 1917 cell and its use in the treatment of Crohn's disease.

Description

technical field [0001] The invention relates to a recombinant Escherichia coli (E. coli) strain Nissle1917 (EcN) cell transformed with nucleic acid encoding defensin protein or its derivative. The present invention also relates to a pharmaceutical composition comprising the cells and a pharmaceutically acceptable carrier, the present invention also relates to a method for preparing recombinant Escherichia coli (E.coli) Nissle1917 cells, and its use in the treatment of Crohn's disease Applications. Background technique [0002] Escherichia coli strain Nissle1917 (EcN) is one of the most thoroughly studied probiotic (Ardeypharm GmbH, Herdecke, Germany). The probiotic drug has been successfully used in many European countries to treat various gastrointestinal diseases, including acute and long-term diarrhea, uncomplicated diverticular disease, and inflammatory bowel disease (IBD). It is particularly useful in maintenance therapy for the relief of symptoms in patients with u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/21C12R1/19C07K14/47A61K35/74
CPCC07K2319/036C07K2319/21C07K14/4723A61K35/74A61K38/1729A61K2035/11
Inventor 托比亚斯·奥尔施拉格尔E·J·徐简·韦坎普爱德华·F·施坦格乌尔里希·索南伯恩于尔根·马林卡汉斯·普罗珀特
Owner 医药中心有限公司
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