Preparation method and application of Antrodia camphorate mycelium medium

A technology of mycelium of Antrodia camphorata and culture medium formula, applied in the field of biotechnology

Active Publication Date: 2014-11-05
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After cooling the culture medium, add Antrodia camphorata strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select Juncao Ganoderma lucidum lees that are free from diseases and insect pests and rich in Ganoderma lucidum mycelium. After breaking the bag, crushing, and drying, weigh 20g and dissolve it in 1000mL of water. Concentrate to 30 mL, freeze at -80°C and put it into a vacuum drying oven to obtain a tan powdery solid, which is the Juncao Ganoderma lucidum extract.

[0023] Preparation of Antrodia antrodia mycelium medium in this example: 2.5% glucose, 0.5% peptone, 0.3% maltose, 0.3% yeast extract, 0.1% KH 2 PO 4 , 0.1% MgSO 4 ·7H 2 O, VB 1 Dissolve 0.1% and 0.1% Juncao Ganoderma lucidum extracts in 1000mL water, adjust the pH value to 5.5, fill each 100mL into 250mL volumetric flasks, sterilize at 121°C for 20min, and cool down for later use.

[0024] Culture method: culture at 28°C, 120rpm, and dark for 16 days.

[0025] After measurement, the growth of mycelium was 8.67g / L (DW), the content of polysaccharide was 0.97%, and the content of triterpene was 0.28...

Embodiment 2

[0028] Select Juncao Ganoderma lucidum lees that are free from diseases and insect pests and rich in Ganoderma lucidum mycelium. After breaking the bag, crushing and drying, weigh 20g and dissolve it in 1000mL of water. Concentrate to 40 mL, freeze at -80°C and put it into a vacuum drying oven to obtain a tan powdery solid, which is the Juncao Ganoderma lucidum extract.

[0029] Preparation of Antrodia antrodia mycelium medium in this example: 3.0% glucose, 1.0% peptone, 1.0% maltose, 1.0% yeast extract, 0.3% KH 2 PO 4 , 0.3% MgSO 4 ·7H 2 O, VB 1 0.1% and 0.5% Juncao Ganoderma lucidum extracts were dissolved in 1000mL water, the pH value was adjusted to 5, each 100mL was dispensed into 250mL volumetric flasks, and sterilized at 121°C for 20min. After the culture medium was cooled, the strains of Antrodia camphorata were added.

[0030] Culture method: culture at 28°C, 120rpm, and dark for 16 days.

[0031] After measurement, the mycelium growth amount was 9.2g / L (DW...

Embodiment 3

[0034] Select Juncao Ganoderma lucidum lees that are free from diseases and insect pests and rich in Ganoderma lucidum mycelium. After breaking the bag, crushing, and drying, weigh 20g and dissolve it in 1000mL of water. Concentrate to 20 mL, freeze at -80°C and put it into a vacuum drying oven to obtain a tan powdery solid, which is the Juncao Ganoderma lucidum extract.

[0035] Preparation of Antrodia antrodia mycelium medium in this example: 1.0% glucose, 0.5% peptone, 0.5% maltose, 0.5% yeast extract, 0.2% KH 2 PO 4 , 0.2% MgSO 4 ·7H 2 O, VB 1 0.1% and 0.1% Juncao Ganoderma lucidum extracts were dissolved in 1000mL of water, the pH value was adjusted to 5.5, each 100mL was dispensed into 250mL volumetric flasks, and sterilized at 121°C for 20min. After the culture medium was cooled, the strains of Antrodia camphorata were added.

[0036] Culture method: culture at 28°C, 120rpm, and dark for 16 days.

[0037] After measurement, the growth of mycelium is 9.0g / L (DW),...

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Abstract

The invention discloses a preparation method and an application of an Antrodia camphorate mycelium medium. The Antrodia camphorate mycelium medium is a liquid medium prepared by mixing a Grass-cultivated Ganodema lucidum extrct with glucose, maltose, a yeast extract, KH2PO4, MgSO4.7H2O and VB1 according to a certain ratio. The Antrodia camphorate mycelium medium enables Antrodia camphorate mycelia to have the characteristics of fast germination, large quantity and rapid growth, enables the polysaccharide content and the triterpene content of the mycelia to reach 0.43-0.97% and 0.2-0.41% respectively and be two times that of mycelia cultivated by traditional mediums, and provides a new method for providing a large amount of Antrodia camphorate mycelia in practical production. The preparation method of the Antrodia camphorate mycelium medium has the advantages of simplicity, strong repeatability, and simple and easy operation.

Description

technical field [0001] The invention relates to a preparation method and application of antrodia mycelium culture medium, belonging to the field of biotechnology. Background technique [0002] Antrodia Cinnamomea ( Antrodia cinnamomea ) is a unique medicinal fungus in Taiwan, my country. It has many functions such as liver protection, detoxification, anti-inflammation, and anti-oxidation. Cinnamomum kanehirai ) is the only host of Antrodia cintrodia. Due to the very small amount of its host in nature and the slow growth rate of Antrodia cintrodia, Antrodia cintrodia is known as "the ruby ​​in the forest". Studies have shown that the mycelium of Antrodia Cinnamomea has a very similar health care function to fruiting bodies of Antrodia Cinnamomea. In order to meet the growing demand of people for Antrodia Cinnamomea, the present invention screens Juncao Ganoderma lucidum extract, glucose, maltose, etc. Rapidly stimulating the growth of mycelia of Antrodia cinnamomea, increas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 李晶林占熺谢长海罗红建林冬梅童金华王培丹邵恩斯
Owner FUJIAN AGRI & FORESTRY UNIV
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