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Liquid stable enzymic method homocysteine kit

A homocysteine ​​and kit technology, applied in the field of medical testing, can solve the problems of limited application of kits, poor thermal stability and poor stability of coupled enzymes, achieve unlimited application range, and improve stability High stability and high effect

Active Publication Date: 2014-11-12
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Although the current enzymatic kits are widely used clinically, due to the poor stability of the substrate SAM (S-adenosylmethionine, S-adenosyl-L-methionine), the coupling enzyme used is thermally stable. Due to various reasons such as poor sex, the clinical application of the kit is limited

Method used

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  • Liquid stable enzymic method homocysteine kit
  • Liquid stable enzymic method homocysteine kit
  • Liquid stable enzymic method homocysteine kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] NADH circulatory system:

[0044] NADH0.3g / L

[0045] Glucose dehydrogenase: 30KU / L

[0046] D-glucose: 25mM

Embodiment 2

[0048] SAM polyanion system:

[0049] SAM sodium polystyrene sulfonate 10mM

[0050] Methyl β-cyclodextrin 15mM

[0051] Mannitol 5g / L

Embodiment 3

[0053] Hcy kit:

[0054] R1:

[0055]

[0056] R2

[0057]

[0058] The method for detecting the Hcy concentration of the sample using the above kit is shown in Table 1 below:

[0059] Table 1

[0060]

[0061]

[0062] The kit prepared by adopting this patent has good stability, and only 5% loss is found in the damage test of 4 degrees centigrade for one year and 37 degrees centigrade for 7 days, fully meeting the clinical needs.

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Abstract

The invention discloses a liquid stable enzymic method homocysteine kit. The liquid stable enzymic method homocysteine kit is characterized by comprising (1) a stable substrate system and (2) stable S-adenosyl-L-homocysteine hydrolase, wherein the stable substrate system comprises an NADH circulatory system and an S-adenosyl methionine polyanion system, and the stable S-adenosyl-L-homocysteine hydrolase improves the overall stability of the kit. By the adoption of the liquid stable enzymic method homocysteine kit, a stable system of S-adenosyl methionine and reduced coenzyme I is provided, the patented technology of enzyme is introduced so that the overall stability of the kit can be improved, and therefore the demand of clinical examination for the liquid stable enzymic method homocysteine kit is met; in addition, the defect that in the prior art, the clinical application of a kit is limited due to the fact that the stability of the substrate S-adenosyl methionine (SAM) is poor and the thermal stability of an adopted coupling enzyme is poor is overcome.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a highly stable liquid-stabilized enzymatic homocysteine ​​kit used in medical testing. Background technique [0002] Homocysteine ​​(Hcy) is the latest monitoring index of independent cardiovascular and cerebrovascular diseases, which can be used to predict the risk of cerebrovascular diseases, and is related to senile dementia, renal failure, miscarriage and premature death from diabetes, so the in vitro Detection has important clinical value. [0003] At present, the enzyme cycle method is mainly used to detect Hcy in plasma. This method can be carried out on an automatic biochemical analyzer. It has high detection sensitivity and is widely used in clinical testing. Its reaction principle is as follows: [0004] [0005] L-Met: L-methionine [0006] SAMT: S-adenosylmethionine transferase (salicylic acid carboxyl methyltransferase) [0007] SAH: S-Adenosyl Homocy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/48C12Q1/34C12Q1/32
Inventor 邹炳德邹继华贾江花章玉胜
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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