A multi-channel parallel detection surface plasmon resonance biosensor and its preparation and detection method

Abstract

The invention discloses a multichannel parallel detection surface plasma resonance (SPR) biosensor, and preparation and detection methods thereof. A sensing unit of the sensor comprises a triangular prism and a gold thin film lattice chip prepared on an optical slide, and cross-linked molecular layers and bioprobe molecules are assembled on the surfaces of lattice points; a halogen tungsten lamp, a precision monochrometer, a spherical lens, a diaphragm, a plano-convex lens and a polarizing film are arranged on an incident light path of the sensor in sequence; a convex lens and a CCD (charge coupled device) video camera are arranged on an emergent light path of the sensor in sequence, and the CCD video camera and the precision monochrometer are connected with a computer. According to a system, the SPR spectrum resonance curves of the lattice points are obtained in parallel by an optical sweeping mode, different biological targets are detected in parallel according to the motion of the SPR peaks of the lattice points, and the sensor has single-channel SPR sensitivity while being used for multichannel parallel detection.

Description

technical field [0001] The invention belongs to the field of biosensors, in particular to a multi-channel parallel detection surface plasmon resonance biosensor and a preparation and detection method thereof. Background technique [0002] Surface plasmon resonance (SPR) is a physical optical phenomenon produced by the coupling of light beams through the surface of a medium and metal thin films. This phenomenon was first discovered by Wood in optical experiments in 1902. Total reflection will occur when light is irradiated from the optically denser medium to the optically rarer medium. If a layer of 45-50nm thick gold film is coated on the interface where total reflection occurs, when the light is incident on the gold layer at an angle greater than the critical angle from the optically denser side of the medium The interface of the gold film will generate evanescent waves on the other side of the interface, and this evanescent wave can cause free electrons in the gold film t...

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Problems solved by technology

However, the above-mentioned SPR technology also has the following disadvantages: only one sample can be detected at a time, so the detection efficiency is low, and high-throughput detection cannot be achieved

Although this device has high sensitivity, its anti-interference ability is very weak, and it is difficult to debug the instrument. The working environment of the instrument is very demanding, and a slight vibration will seriously interfere with the test results.

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