Antifreeze agent and diluent for livestock semen refrigeration preservation and application thereof

A technology for cryopreservation and dilution, which is applied in the application, preservation of human or animal body, animal husbandry, etc., to achieve the effects of simple preparation method, improved sperm motility rate, and reliable effect.

Inactive Publication Date: 2014-11-26
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are no relevant reports at home and abroad on the study of betaine as a cryoprotectant for the cryopreservation of cattle, pigs and other livestock semen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Accurately measure conventional diluent components (glucose 1.1g, citric acid 1.48g, Tris 2.42g, fresh egg yolk 10% (volume), penicillin sodium 0.06g, streptomycin sulfate 0.1g) dissolved in 100ml distilled water, add beet Alkali 100-200mg, glycerol 3% is formulated into the diluent for pig semen cryopreservation. The pH of the solution was adjusted to 6.5-7.5, sterilized by filtration, cooled to room temperature, and placed in a refrigerator at 4°C for later use.

[0026] The inventor conducted the following tests on the effect of using the diluent for cryopreservation of livestock semen in this embodiment.

[0027] (1) Semen collection

[0028] The semen was collected by the hand holding method, and the thick part of the semen was selected, filtered through 4 layers of sterile gauze to remove jelly, and routine quality inspection was carried out at 37°C with a microscope. Select semen with no peculiar smell, milky white color, normal sperm morphology, and a motility...

Embodiment 2

[0045] Accurately measure conventional diluent components (glucose 1.1g, citric acid 1.48g, Tris 2.42g, fresh egg yolk 10% (volume), penicillin sodium 0.06g, streptomycin sulfate 0.1g) dissolved in 100ml distilled water, add beet 150-250 mg of alkali and 4% (volume) of glycerol are prepared into a diluent for frozen preservation of bovine semen. The pH of the solution was adjusted to 6.5-7.5, sterilized by filtration, cooled to room temperature, and placed in a refrigerator at 4°C for later use.

[0046] The inventor conducted the following tests on the effect of using the diluent for cryopreservation of livestock semen in this embodiment.

[0047] (1) Semen collection

[0048] Semen was collected by the pseudo-vaginal method, and routine quality inspection was carried out at 37°C with a microscope. Select semen with no peculiar smell, milky white color, normal sperm morphology, motility rate above 0.7, and "dense" density for the test.

[0049] (2) Semen processing and freez...

Embodiment 3

[0066] Accurately measure conventional diluent components (glucose 1.1g, citric acid 1.48g, Tris 2.42g, fresh egg yolk 10% (volume), penicillin sodium 0.06g, streptomycin sulfate 0.1g) dissolved in 100ml distilled water, add beet 50-100 mg of alkali and 2% (volume) of glycerol are prepared to prepare a diluent for cryopreservation of goat semen. The pH of the solution was adjusted to 6.5-7.5, sterilized by filtration, cooled to room temperature, and placed in a refrigerator at 4°C for later use.

[0067] The inventor conducted the following tests on the effect of using the diluent for cryopreservation of livestock semen in this embodiment.

[0068] (1) Semen collection

[0069] Semen was collected by the pseudo-vaginal method, and routine quality inspection was carried out at 37°C with a microscope. Select semen with no peculiar smell, milky white color, normal sperm morphology, motility rate above 0.7, and "dense" density for the test.

[0070] (2) Semen processing and fre...

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PUM

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Abstract

The invention discloses an antifreeze agent and a diluent for livestock semen refrigeration preservation and application thereof. The antifreeze agent for livestock semen refrigeration preservation is betaine. The diluent for livestock semen refrigeration preservation includes a conventional diluent and an antifreeze agent, the antifreeze agent is betaine, and the conventional diluent formula is preferred as follows: 1.1g of glucose, 1.48g of citric acid, 2.42g of Tris, 10% of fresh egg yolk, 0.06g of penicillin sodium, 0.1g of streptomycin sulfate and 100ml of distilled water. The antifreeze agent for livestock semen refrigeration preservation is especially suitable for artificial insemination of cattle, pigs, sheep tubule frozen semen, and semen deposition after long-term storage and long-distance transportation of super-low temperature frozen semen, sperm motility rate, perforatorium integrity rate and plasmalemma integrity rate after freezing-thawing can be significantly improved, and the sperm fertilization vitality can be maintained.

Description

technical field [0001] The invention belongs to the technical field of ultra-low temperature cryopreservation of mammalian semen, and specifically relates to an antifreeze agent for cryopreservation of livestock semen, and also relates to a diluent for cryopreservation of livestock semen, and further relates to the application of the diluent for cryopreservation of livestock semen. Background technique [0002] Mammalian semen cryopreservation technology provides important technical support for the development of highly intensive modern animal husbandry. Since glycerol was used as a cryoprotectant for semen cryopreservation, countries all over the world have started large-scale research and application. Semen cryopreservation can last for decades, which solves the problem of protection of mammalian male germplasm resources, makes the use of male germplasm resources not limited by time and region, and facilitates inter-provincial and international cooperation , to improve th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 李青旺胡靓华江中良
Owner NORTHWEST A & F UNIV
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