A kind of biological cartilage repair material and its preparation method
A cartilage repair and animal technology, applied in the field of medical devices, can solve the problems of limited source of autologous cartilage, cross infection, immune rejection, etc., and achieve the effect of being conducive to regenerative repair, no immune rejection, and good biocompatibility
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Embodiment 1
[0034] Embodiment 1, the preparation of biotype cartilage repair material
[0035] 1. Collect the costal cartilage of adult pigs that have just been slaughtered from slaughterhouses under standardized management, try to avoid contact with pollutants, and freeze them immediately after collection.
[0036] 2. Thaw the costal cartilage obtained in step 1 and wash it thoroughly, cut off the muscle tissue, and remove the periosteum (for the results of HE staining at this time, see figure 1 ).
[0037] 3. Decellularization treatment
[0038] Treat the costal cartilage by immersion in a surfactant solution.
[0039] The purpose of this step is to destroy the cell membrane structure and break up the cells for dissolution.
[0040] Specific steps: use 0.5g / 100ml TritonX-100 aqueous solution to soak for 15 hours at 8±2°C, and then wash with water.
[0041] 4. The first alkali treatment
[0042] Use alkaline solution to soak the product of step 3.
[0043] The purpose of this step is...
Embodiment 2
[0056] Embodiment 2, the preparation of biotype cartilage repair material
[0057] 1. Collect the articular cartilage of adult pigs that have just been slaughtered from slaughterhouses under standardized management, avoid contact with pollutants as much as possible, and freeze them immediately after collection.
[0058] 2. The articular cartilage obtained in step 1 is thawed and fully cleaned, the muscle tissue is cut off, and the periosteum is removed.
[0059] 3. Decellularization treatment
[0060] Treatment of articular cartilage by soaking in a surfactant solution.
[0061] Specific steps: Soak with 1g / 100ml Tween-80 aqueous solution at 23±2°C for 5 hours, then wash with water.
[0062] 4. The first alkali treatment
[0063] Use alkaline solution to soak the product of step 3.
[0064] Specific steps: use 0.5M KOH aqueous solution, soak at 23±2°C for 30 minutes, and then wash with water.
[0065] 5. Degreasing treatment
[0066] Soak the product of step 4 with a sur...
Embodiment 3
[0074] Embodiment 3, the preparation of biological cartilage repair material
[0075] 1. Collect the costal cartilage of adult cattle that have just been slaughtered from slaughterhouses under standardized management, avoid contact with pollutants as much as possible, and freeze and store immediately after collection.
[0076] 2. Thaw the costal cartilage obtained in step 1 and fully wash it, cut off the muscle tissue, and remove the periosteum.
[0077] 3. Decellularization treatment
[0078] Treat the costal cartilage by immersion in a surfactant solution.
[0079] Specific steps: Soak in 3g / 100ml Tween-40 aqueous solution at 2±2°C for 168 hours, then wash with water.
[0080] 4. The first alkali treatment
[0081] Use alkaline solution to soak the product of step 3.
[0082] Specific steps: use 0.02MCa(OH) 2 Aqueous solution, soak at 2±2°C for 180min, then wash with water.
[0083] 5. Degreasing treatment
[0084] Soak the product of step 4 with a surfactant solution...
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