A kind of dense bone matrix and its preparation method
A technology of bone matrix and immersion treatment, applied in the field of medical devices, can solve the problems of difficulty in storage, non-degradation, and inability to achieve strength, and achieve the effects of good biocompatibility, soft surface, and avoidance of secondary damage
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Embodiment 1
[0046] Example 1. Preparation of dense bone matrix (completely decalcified)
[0047] 1. Collect limb bones of adult pigs that have just been slaughtered from standardized management slaughterhouses, avoid contact with pollutants as much as possible, and freeze them immediately after collection.
[0048] 2. Thaw and fully wash the limb bone obtained in step 1, take the backbone part, remove the periosteum and bone marrow, and process it into strips or blocks suitable for handling.
[0049] 3. Decellularization treatment
[0050] Treat the backbone with a surfactant solution soak.
[0051] The purpose of this step is to destroy the cell membrane structure and break up and dissolve the cells.
[0052] Specific steps: soak in 0.5g / 100ml TritonX-100 aqueous solution at 8±2°C for 12 hours, and then wash with water.
[0053] 4. The first alkali treatment
[0054] Use alkaline solution to soak the product of step 3.
[0055] The purpose of this step is to destroy and dissolve non...
Embodiment 2
[0074] Example 2. Preparation of dense bone matrix (completely decalcified)
[0075] 1. Collect limb bones of adult cattle that have just been slaughtered from slaughterhouses under standardized management, try to avoid contact with pollutants, and freeze them immediately after collection.
[0076] 2. Thaw and fully wash the limb bone obtained in step 1, take the backbone part, remove the periosteum and bone marrow, and process it into strips or blocks suitable for handling.
[0077] 3. Decellularization treatment
[0078] Treat the backbone with a surfactant solution soak.
[0079] Specific steps: Soak with 1g / 100ml Tween-80 aqueous solution at 23±2°C for 1 hour, and then wash with water.
[0080] 4. The first alkali treatment
[0081] Use alkaline solution to soak the product of step 3.
[0082] Specific steps: use 0.02MCa(OH) 2 Aqueous solution, soak at 2±2°C for 180min, then wash with water.
[0083] 5. Degreasing treatment
[0084] Soak the product of step 4 with a...
Embodiment 3
[0097] Example 3. Preparation of dense decalcified bone matrix (completely decalcified)
[0098] 1. Collect limb bones of adult pigs that have just been slaughtered from standardized management slaughterhouses, avoid contact with pollutants as much as possible, and freeze them immediately after collection.
[0099]2. Thaw and fully wash the limb bone obtained in step 1, take the backbone part, remove the periosteum and bone marrow, and process it into strips or blocks suitable for handling.
[0100] 3. Decellularization treatment
[0101] Treat the backbone with a surfactant solution soak.
[0102] Specific steps: Soak in 3g / 100ml Tween-40 aqueous solution at 2±2°C for 168 hours, then wash with water.
[0103] 4. The first alkali treatment
[0104] Use alkaline solution to soak the product of step 3.
[0105] Specific steps: use 0.5M KOH aqueous solution, soak at 23±2°C for 30 minutes, and then wash with water.
[0106] 5. Degreasing treatment
[0107] Soak the product o...
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