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A kind of natural inorganic bone matrix and preparation method thereof

A bone matrix and inorganic technology, which is applied in the field of natural inorganic bone matrix and its preparation, can solve the problems of patients' postoperative quality of life, insufficient porosity, insufficient strength, etc., and achieves no immune rejection, low mechanical strength, sufficient The effect of mechanical strength

Active Publication Date: 2015-10-14
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problem of metal materials as bone graft materials is their thermal conductivity, which affects the quality of life of patients after surgery
The commonly used inorganic non-metallic materials (such as bone cement, calcium phosphate ceramics, etc.) have problems such as insufficient porosity, insufficient strength, and poor operability.
Existing natural inorganic bone matrix suffers from insufficient porosity and insufficient strength

Method used

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  • A kind of natural inorganic bone matrix and preparation method thereof
  • A kind of natural inorganic bone matrix and preparation method thereof
  • A kind of natural inorganic bone matrix and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the preparation of natural inorganic bone matrix

[0033] 1. Preparation of natural inorganic bone matrix

[0034] 1. Preparation of spongy bone

[0035] (1) Collect fresh long bones of pigs that have just been slaughtered from standardized management slaughterhouses, avoid contact with pollutants as much as possible, and freeze them immediately after collection.

[0036] (2) The long bone obtained in step (1) is thawed and fully cleaned, the cancellous bone is cut out, and then the cancellous bone is cut into a form that is easy to handle, and the surface attachments are washed away.

[0037] 2. Preparation of natural inorganic bone matrix

[0038] (1) Decellularization treatment

[0039] Treat spongy bone with a surfactant solution soak.

[0040] The purpose of this step is to destroy the cell membrane structure, break and dissolve the cells, and remove most of the fat and fat-soluble impurities in the bone tissue.

[0041]Specific steps: Soak in 0....

Embodiment 2

[0076] Embodiment 2, the preparation of natural inorganic bone matrix

[0077] 1. Preparation of spongy bone

[0078] (1) Collect fresh long bones of cattle that have just been slaughtered from standardized management slaughterhouses, avoid contact with pollutants as much as possible, and freeze them immediately after collection.

[0079] (2) The long bone obtained in step (1) is thawed and fully cleaned, the cancellous bone is cut out, and then the cancellous bone is cut into a form that is easy to handle, and the surface attachments are washed away.

[0080] 2. Preparation of natural inorganic bone matrix

[0081] (1) Decellularization treatment

[0082] Treat spongy bone with a surfactant solution soak.

[0083] Specific steps: Soak in 2g / 100ml Tween-80 aqueous solution at 28±2°C for 5 hours, then wash with water.

[0084] (2) The first alkali treatment

[0085] The product of step (1) is soaked in an alkaline solution.

[0086] Specific steps: Use 1M KOH aqueous solu...

Embodiment 3

[0106] Embodiment 3, the preparation of natural inorganic bone matrix

[0107] 1. Preparation of spongy bone

[0108] (1) Collect fresh long bones of pigs that have just been slaughtered from standardized management slaughterhouses, avoid contact with pollutants as much as possible, and freeze them immediately after collection.

[0109] (2) The long bone obtained in step (1) is thawed and fully cleaned, the cancellous bone is cut out, and then the cancellous bone is cut into a form that is easy to handle, and the surface attachments are washed away.

[0110] 2. Preparation of natural inorganic bone matrix

[0111] (1) Decellularization treatment

[0112] Treat spongy bone with a surfactant solution soak.

[0113] Specific steps: Soak in 1g / 100ml Tween-80 aqueous solution at 6±2°C for 168 hours, then wash with water.

[0114] (2) The first alkali treatment

[0115] The product of step (1) is soaked in an alkaline solution.

[0116] Specific steps: Use 0.5M NaOH aqueous so...

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Abstract

The invention discloses a natural inorganic bone matrix and its preparation method. The method for preparing the inorganic bone matrix comprises the following steps: (1) a surfactant solution is used to immerse and process cancellous bone; (2) aqueous alkali is used to immerse and process a product obtained from the step (1); (3) gradient high-temperature processing is carried out on a product obtained from the step (2); (4) aqueous alkali is used to immerse and process a product obtained from the step (3); and (5) a PBS buffer solution is used to immerse and process a product obtained from the step (4) so as to obtain the inorganic bone matrix. The gradient high-temperature processing comprises the following steps: (1) calcining is carried out at 105-110 DEG C for 1.5-3 hours; (2) calcining is carried out at 350-400 DEG C for 1.5-3 hours; (3) calcining is carried out at 750-800 DEG C for 3-5 hours; and (4) calcining is carried out at 1200-1300 DEG C for 1.5-3 hours. Adopted for bone transplantation, the inorganic bone matrix has advantages of no immunological rejection, good biocompatibility and enough mechanical strength, and can meet mechanical requirements of repaired tissues.

Description

technical field [0001] The invention relates to a natural inorganic bone matrix and a preparation method thereof. Background technique [0002] In modern surgery, it is often necessary to repair bone tissue. For a long time, the material mainly adopts autologous or allogeneic bone graft. [0003] There are serious defects in the material source of autologous bone transplantation. Taking bone from autologous heterotopia is tantamount to tearing down the east wall to fill the west wall, making patients prone to postoperative complications, and the failure rate is as high as 10%-30%. [0004] Allogeneic bone transplantation is quite difficult in terms of material selection and storage, and is expensive. It is also prone to immune rejection, HIV infection, etc., and has a higher failure rate. At the same time, allograft bone is replaced slowly, and the volume of new bone is relatively small. [0005] In order to overcome various problems existing in autologous bone and allogr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36
Inventor 孙先昌董佳桓林萍马百聚郭松蒋绍霞
Owner YANTAI ZHENGHAI BIO TECH
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