Compact bone matrix and preparation method

A bone matrix, animal bone technology, applied in the field of medical devices, can solve the problems of non-degradability, difficult storage, high failure rate, and achieve the effect of soft surface, avoiding secondary damage and good biocompatibility

Active Publication Date: 2014-12-03
YANTAI ZHENGHAI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the rapid development of surgical technology, it is relatively difficult to obtain suitable repair materials, which has become a major obstacle to the promotion of this type of surgery
At present, many metal repair materials are used, which have the advantages of light weight, high strength, and convenient shaping, but there are still the following problems: the thermal conductivity is too good, causing a strong foreign body sensation to the patient; the elastic modulus is much stronger than that of real bone, Easy to cause mechanical damage to the surrounding bone tissue; non-degradable, existing in the body as a permanent foreign body, and non-bacterial inflammatory reactions often occur
The inorganic non-metallic materials developed later, represented by bioceramics, are superior to metal materials in terms of biocompatibility, but their density is too large, which is subject to certain restrictions in application, and such materials are not easy to degrade, even if they can be well compatible with tissues. Tolerance, can not achieve the desired strength
Autologous bone is the most ideal repair material. It cannot be used in large quantities due to source limitations. It only has excellent performance in the treatment of nonunion, but it will also cause new damage and increase the occurrence of postoperative complications. The failure rate is as high as 10-30% %
Allogeneic bone transplantation is quite difficult and expensive in terms of material selection and storage, and it is also prone to immune rejection, HIV infection, etc., and has a higher failure rate

Method used

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  • Compact bone matrix and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1. Preparation of dense bone matrix (completely decalcified)

[0047] 1. Collect limb bones of adult pigs that have just been slaughtered from standardized management slaughterhouses, avoid contact with pollutants as much as possible, and freeze them immediately after collection.

[0048] 2. Thaw and fully wash the limb bone obtained in step 1, take the backbone part, remove the periosteum and bone marrow, and process it into strips or blocks suitable for handling.

[0049] 3. Decellularization treatment

[0050] Treat the backbone with a surfactant solution soak.

[0051] The purpose of this step is to destroy the cell membrane structure and break up and dissolve the cells.

[0052] Specific steps: Soak in 0.5g / 100ml TritonX-100 aqueous solution at 8±2°C for 12 hours, then wash with water.

[0053] 4. The first alkali treatment

[0054] Use alkaline solution to soak the product of step 3.

[0055] The purpose of this step is to destroy and dissolve non-col...

Embodiment 2

[0074] Example 2. Preparation of dense bone matrix (completely decalcified)

[0075] 1. Collect limb bones of adult cattle that have just been slaughtered from slaughterhouses under standardized management, try to avoid contact with pollutants, and freeze them immediately after collection.

[0076] 2. Thaw and fully wash the limb bone obtained in step 1, take the backbone part, remove the periosteum and bone marrow, and process it into strips or blocks suitable for handling.

[0077] 3. Decellularization treatment

[0078] Treat the backbone with a surfactant solution soak.

[0079] Specific steps: Soak with 1g / 100ml Tween-80 aqueous solution at 23±2°C for 1 hour, and then wash with water.

[0080] 4. The first alkali treatment

[0081] Use alkaline solution to soak the product of step 3.

[0082] Specific steps: use 0.02M Ca(OH) 2 Aqueous solution, soak at 2±2°C for 180min, then wash with water.

[0083] 5. Degreasing treatment

[0084] Soak the product of step 4 with ...

Embodiment 3

[0097] Example 3. Preparation of dense decalcified bone matrix (completely decalcified)

[0098] 1. Collect limb bones of adult pigs that have just been slaughtered from standardized management slaughterhouses, avoid contact with pollutants as much as possible, and freeze them immediately after collection.

[0099] 2. Thaw and fully wash the limb bone obtained in step 1, take the backbone part, remove the periosteum and bone marrow, and process it into strips or blocks suitable for handling.

[0100] 3. Decellularization treatment

[0101] Treat the backbone with a surfactant solution soak.

[0102] Specific steps: Soak with 3g / 100ml Tween-40 aqueous solution at 2±2°C for 168 hours, and then wash with water.

[0103] 4. The first alkali treatment

[0104] Use alkaline solution to soak the product of step 3.

[0105] Specific steps: use 0.5M KOH aqueous solution, soak at 23±2°C for 30 minutes, and then wash with water.

[0106] 5. Degreasing treatment

[0107] Soak the prod...

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Abstract

The invention discloses a compact bone matrix and its preparation method. The method for preparing the bone matrix (a compact demineralized bone matrix) comprises the following steps: (1) diaphysis of isolated animal bones is taken and is immersed and processed by the use of a surfactant solution; (2) a product obtained from the step (1) is immersed and processed by the use of aqueous alkali; (3) a product obtained from the step (2) is immersed and processed by the use of a surfactant solution; (4) a product obtained from the step (3) is immersed and processed by the use of an acid solution; (5) a product obtained from the step (4) is immersed and processed by the use of aqueous alkali; (6) a product obtained from the step (5) is immersed and processed by the use of an irradiation protective reagent solution with the pH of 6.0-7.5; and (7) a product obtained from the step (6) undergoes freeze drying and irradiation sterilization successively so as to obtain the bone matrix. The bone matrix provided by the invention has advantages of no immunological rejection and good biocompatibility, and can meet mechanical requirements of repaired tissues.

Description

technical field [0001] The invention belongs to the field of medical instruments, and in particular relates to a dense bone matrix for repairing bone tissue in surgical operations and a preparation method thereof. Background technique [0002] With the development of modern medicine, the scope of orthopedic plastic surgery has become more and more extensive, such as repair of comminuted fractures and nonunions, skull reconstruction, maxillofacial plastic surgery, spinal repair and reconstruction, etc. [0003] With the rapid development of surgical technology, it is relatively difficult to obtain suitable repair materials, which has become a major obstacle to the promotion of this type of surgery. At present, many metal repair materials are used, which have the advantages of light weight, high strength, and convenient shaping, but there are still the following problems: the thermal conductivity is too good, causing a strong foreign body sensation to the patient; the elastic ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 董佳桓孙先昌马百聚王丽林萍
Owner YANTAI ZHENGHAI BIO TECH
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