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Combined chromatographic column and column chromatography method for ingredient separation of traditional Chinese medicine/natural medicine

A chromatographic column and combined technology, applied in separation methods, chemical instruments and methods, solid adsorbent liquid separation, etc., can solve the problems of time-consuming, high elution cost, and achieve rapid separation and enrichment with good reproducibility. Effect

Inactive Publication Date: 2014-12-17
成都草源康生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the target component or component group is eluted first, no further elution is required in the later stage; but if the impurity is eluted first, then a large amount of mobile phase is needed to elute the target component or component group later, not only Time consuming and very expensive to elute

Method used

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  • Combined chromatographic column and column chromatography method for ingredient separation of traditional Chinese medicine/natural medicine
  • Combined chromatographic column and column chromatography method for ingredient separation of traditional Chinese medicine/natural medicine
  • Combined chromatographic column and column chromatography method for ingredient separation of traditional Chinese medicine/natural medicine

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Embodiment 1

[0039] Embodiment 1 Chromatography column of the present invention

[0040] Such as Figure 1~3 As shown, the present invention actually provides a combined chromatographic column, which is composed of at least 2 stationary phase filling tubes 1, and every two stationary phase filling tubes 1 are connected up and down through the sealing holder 2; There is a drain valve 3 at the bottom of the stationary phase filling tube 1 at the end.

[0041] The above-mentioned chromatographic column combines several sections of stationary phase filling tubes into a whole through a sealed retainer. The chromatographic column of the present invention is used for separation. Firstly, the sample is loaded according to the conventional column chromatography technology, and then a certain mobile phase is used for elution, and the bottom drain valve is closed in due course. The column containing the components or component groups to be separated is disassembled, and the The adsorbent (i.e. the ...

Embodiment 2

[0054] Embodiment 2 chromatography method of the present invention

[0055] In this embodiment, the research on the substance basis of magnolia officinalis for promoting gastrointestinal motility is used as an example for illustration.

[0056] 1. Column chromatography operation

[0057] (1) Sample preparation

[0058] The medicinal materials collected in HK Township, Dujiangyan (collection number DJYHK20130930) were extracted by percolation method with 95% ethanol as a solvent, and 100g of the extract was taken separately, mixed with diatomaceous earth, and set aside.

[0059] (2) Column chromatography operation

[0060] Take the chromatographic column in the embodiment, wherein there are 3 stationary phase filling tubes (1), and after the chromatographic column is assembled, it is set aside;

[0061] Column packing: silica gel wet packing;

[0062] Dry sample loading: pour the spin-dried sample gently into the chromatographic column with the help of a glass rod, try to a...

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Abstract

The invention discloses a combined chromatographic column and a column chromatography method based on the same. The combined chromatographic column is composed of at least two stationary phase filling tubes in a combined mode, and each two adjacent stationary phase filling tubes are vertically communicated through a sealing fixer; a liquid discharge valve is arranged at the bottom of the lowest stationary phase filling tube. The combined chromatographic column or the column chromatography method can separate stage by stage in the chromatographic separation process so that an operator is convenient to directly separate to take adsorbent with a target ingredient or ingredient group according to actual needs, the elution time is greatly saved, and elution solvent is greatly saved. The column chromatography method provides a new method for exploring the effective ingredients (ingredient groups) of the traditional Chinese medicine.

Description

technical field [0001] The invention relates to a combined chromatographic column and a column chromatographic method for separating traditional Chinese medicine / natural medicine components. Background technique [0002] Column chromatography is a more classic organic separation technology, the principle is the same as thin-layer chromatography, but the device is different, mainly composed of a glass column and packing material. The most common column chromatography is silica gel chromatography, alumina chromatography, ion exchange column chromatography, polyamide column chromatography and gel column chromatography, macroporous resin column chromatography, etc. [0003] In the current chromatographic separation operation, the sample to be separated is loaded, and then the mobile phase is added from the top, and the compound is eluted from the chromatographic column by using the polarity difference between the stationary phase and the mobile phase, so that the separated produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/22
Inventor 龙飞
Owner 成都草源康生物科技有限公司
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