Fertilizer prepared by utilizing threonine fermented wastes

A technology for fermenting waste and threonine fermentation liquid, which is applied in the directions of ammonium orthophosphate fertilizer, alkaline orthophosphate fertilizer, organic fertilizer, etc. The effect of reducing production cost, reducing raw material cost and improving utilization rate

Active Publication Date: 2015-01-07
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Threonine is mainly used as a feed additive, and its production steps are concentration, crystallization, and extraction of bacterial protein. However, the bacterial protein and mother liquor produced in the process are difficult to process and costly, and it is difficult to obtain cost-effective by-products
[0004] The development and application of fertilizers in my country is the main stage from farmyard manure to the use of inorganic fertilizers. Because farmyard manure has many pollution sources and a large amount of transportation, it is time-consuming and laborious to operate and the effect is not particularly obvious; while inorganic fertilizer has a singl

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of fertilizer that utilizes threonine to ferment waste to prepare, it comprises the following steps:

[0025] Step 1) preparation of threonine fermentation broth, step 2) filtration and decolorization, step 3) separation of crystals and mother liquor, step 4) hydrolysis of bacterial protein, step 5) preparation of feed liquid A, and step 6) preparation of fertilizer;

[0026] 1) Preparation of threonine fermentation broth: Escherichia coli engineering bacteria K12△dapA (see "Optimization of E. (Refer to documents such as CN1305009A and CN1284560A) The mixed bacterial solution is put into the seed tank according to 10% inoculation amount for cultivation, at a temperature of 32°C and a shaker speed of 180r / min, cultivated for 12 hours to obtain liquid A; then according to the liquid A: The fermentor culture medium is that the volume ratio of 1: 10 is transferred in the fermentor and cultivated, and temperature is 32 ℃, cultivates 5 days, obtains fermented liquid; T...

Embodiment 2

[0034] A kind of fertilizer that utilizes threonine to ferment waste to prepare, it comprises the following steps:

[0035] Step 1) preparation of threonine fermentation broth, step 2) filtration and decolorization, step 3) separation of crystals and mother liquor, step 4) hydrolysis of bacterial protein, step 5) preparation of feed liquid A, and step 6) preparation of fertilizer;

[0036] 1) Preparation of threonine fermentation broth: Escherichia coli engineering bacteria K12△dapA (see "Optimization of E. (Refer to documents such as CN1305009A and CN1284560A) The mixed bacterial solution is put into the seed tank according to the inoculum amount of 5% for cultivation, at a temperature of 32°C, with a shaker speed of 200r / min, and cultivation for 16 hours to obtain liquid A; then, according to the liquid A: the fermentor culture medium is that the volume ratio of 1: 10 is transferred in the fermentor and cultivated, and the temperature is 32 ℃, cultivates for 5 days, obtains fe...

Embodiment 3

[0044] Fertilizer effect test of fertilizer of the present invention

[0045] 1. Fertilizer experiment for planting sorghum:

[0046] The control group used compound fertilizer (nitrogen-phosphorus-potassium=23-7-6); the experimental group used the fertilizer prepared in Example 1 of the present invention; the soil quality and planting conditions of the test field were exactly the same, and the area was one mu; the amount of fertilizer applied was 80kg / mu. The specific test results are shown in Table 1:

[0047] Table 1

[0048] group Yield per mu (Kg) Production increase (%) Fertilization input (yuan) control group 487.9 / 208 test group 523.1 7.21 168

[0049] Note: The fertilizer for the control group is calculated at 2.6 yuan / kg, and the fertilizer for the experimental group is calculated at 2.1 yuan / kg.

[0050] Second, planting sweet potato fertilizer experiment:

[0051] Set up two treatment test fields, each with an area of ​​on...

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PUM

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Abstract

The invention belongs to the field of fermented waste liquid treatment in the biological fermentation industry and discloses a fertilizer prepared by utilizing threonine fermented wastes. The fertilizer is prepared by the following steps: (1) preparing threonine fermentation liquor; (2) filtering and discoloring; (3) separating crystals and a mother solution; (4) hydrolyzing mycoprotein; (5) preparing feed liquid A; and (6) preparing the fertilizer. According to the fertilizer, amino acid fermented wastes are effectively utilized, the production cost of the fertilizer is reduced, the wastes are changed into valuable materials, the fertilizer efficiency is lasting, and the fertilizer is superior to like products in the market.

Description

technical field [0001] The invention belongs to the field of fermentation waste liquid treatment in the biological fermentation industry, and in particular relates to a fertilizer prepared from threonine fermentation waste. [0002] Background technique [0003] Threonine is an essential amino acid, which is the second limiting amino acid in pig feed and the third limiting amino acid in poultry feed. With the wide application of lysine and methionine synthetic products in compound feed, threonine has gradually become an The main limiting factor for the growth of livestock and poultry. Threonine is mainly used as a feed additive. Its production steps are concentration, crystallization, and extraction of bacterial protein. However, the processing of bacterial protein and mother liquor produced in the process is difficult and costly, and it is difficult to obtain cost-effective by-products. [0004] The development and application of fertilizers in my country is the main stag...

Claims

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Application Information

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IPC IPC(8): C05G1/00
CPCC05B7/00C05C9/00C05F7/00C05F11/00
Inventor 张传森时夫龙卢松朱心双邵风慧徐彩凤梁晓娟
Owner 内蒙古阜丰生物科技有限公司
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