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Preparation method of Talaromyces flavus spore powder, Talaromyces flavus wettable pulvis and preparation method of wettable pulvis

A technology of Talaromyces tularensis and wettable powder, which is applied in the fields of the Talaromyces tularensis wettable powder and preparation thereof, the fermentation and preparation of spore powder of Talaromyces xylophilus, the wettable powder of microbial pesticides and the preparation thereof, and can solve the restriction The use and promotion of Talaromyces yellows has the effect of accelerating the promotion and application process.

Active Publication Date: 2015-01-21
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Talaromyces flavus is one of the important bio-control bacteria. At present, there is no corresponding preparation research and development of this fungus in China, which greatly restricts the use and promotion of Talaromyces flavus in biological control.

Method used

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  • Preparation method of Talaromyces flavus spore powder, Talaromyces flavus wettable pulvis and preparation method of wettable pulvis
  • Preparation method of Talaromyces flavus spore powder, Talaromyces flavus wettable pulvis and preparation method of wettable pulvis
  • Preparation method of Talaromyces flavus spore powder, Talaromyces flavus wettable pulvis and preparation method of wettable pulvis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Conditions for Solid Fermentation of Basilisk Yellow Fungus qw12

[0027] 1. Determine the solid fermentation substrate

[0028] Set up three single-substance culture media: bran, corn stalk powder, and corn flour; four mixed-substance culture media: bran+corn stalk powder, bran+corn meal, corn stalk powder+corn meal, bran + corn stalk powder + corn flour, the different ingredients in the mixed substance medium are mixed in equal proportions. Ratio of material to water = 1:1, sterilized at 121°C for 30 minutes, inoculated with 1×10 6 spore suspension per mL, the inoculum size was 5%; cultured at 28°C for 7 days, and turned over once a day. Three replicates were set up for each treatment. After the fermentation, take 0.5 g of the solid fermentation culture, add sterile water, stir on a magnetic stirrer for 5 min, and count the number of spores under a microscope with a hemocytometer. The results show that when the medium is a single substrate, the effect of bran is t...

Embodiment 2

[0075] Embodiment 2: the preparation of yellow basket-shaped fungus spore powder

[0076] The optimized spore-forming medium of the yellow basket-shaped bacteria was sterilized at 121°C for 20-30 minutes and inoculated for 10 6 Individual / mL spore suspension, cultivated in the dark at 28°C for 10 days. After the fermentation is over, dry the solid medium in an oven at 35°C to dry the water, dry and crush the solid culture and pass it through a 200-mesh sieve; or use a fungal spore separator to separate the spores and pass through a 200-mesh sieve; the collected yellow basket-shaped fungus Conidia powder.

[0077] Weigh 0.05g of the spore powder of the yellow basket-shaped fungus and suspend it in 20mL of sterile water, stir it with a magnetic stirrer for 30 minutes, then use a hemocytometer to measure the concentration of the spore suspension, and calculate the spore content of the yellow basket-shaped fungus conidia powder. Repeat 3 times to calculate the average spore cont...

Embodiment 3

[0081] Embodiment 3: the screening of auxiliary agent

[0082] 1 Carrier screening

[0083] The effect of carrier on the germination of spores of T. jaundice: Kaolin, talcum powder, bentonite, and diatomaceous earth are mixed with PDA medium at a concentration ratio of 5 mg / mL and 10 mg / mL, sterilized, and prepared under sterile conditions flat. Take 1.0×10 3 0.1mL of the spore suspension of the yellow basket bacterium qw12 per ml was spread on the plate containing different carriers, and the PDA medium without carrier was used as the control, and each treatment was repeated 4 times, cultured at 28°C, observed and observed after 48 hours. Count the number of colonies formed by spore germination on plates containing the different carriers.

[0084] The influence of the carrier on the mycelial growth of the yellow basket-shaped fungus: put the yellow basket-shaped fungus qw12 bacterial block with a diameter of 0.5cm in the center of the plate containing different carriers mad...

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Abstract

The invention relates to a Talaromyces flavus wettable pulvis. The Talaromyces flavus wettable pulvis includes Talaromyces flavus conidiospore powder, a carrier and an assistant, the content of living bacteria in the Talaromyces flavus wettable pulvis is 5*10<8>-5*l0<9>cfu / g / g, the suspension rate of the Talaromyces flavus wettable pulvis is greater than 75% wettable powder, the wetting time is less than 60s, and the water content is less than 6%. In the invention, the spore fermentation technology of Talaromyces flavus aw12 strains is optimized; and the Talaromyces flavus wettable pulvis is created by using Talaromyces flavus spores as an active component. The invention provides a production method of a Talaromyces flavus biocontrol preparation, and a new convenient and practical dosage form. The Talaromyces flavus spore powder preparation method lays a foundation for the large scale commercialization production of the Talaromyces flavus wettable pulvis, is in favor accelerating the application process of Talaromyces flavus in the biological control, and is of theoretic and practical significance.

Description

technical field [0001] The present invention relates to the technical field of microbial pesticides, in particular to a wettable powder of microbial pesticides and a preparation method thereof, more specifically, the present invention relates to a method for fermenting and preparing spore powder of yellow basket-shaped fungus, and the yellow basket-shaped fungus can be Wet powder and its preparation method. Background technique [0002] Pesticides play an important role in preventing and controlling plant diseases and insect pests, ensuring stable and increased agricultural production, improving the quality of agricultural products, and improving people's living standards. Chemical pesticides have played an important role in controlling crop diseases and protecting agricultural safety, but they have also caused a series of environmental and social problems, such as the "3R" problem caused by the widespread use of chemical pesticides, etc., seriously affecting the sustainabil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/04A01P3/00C12N3/00C12R1/645
Inventor 咸洪泉张军霞李雅华
Owner QINGDAO AGRI UNIV
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