Method for extracting mignonettetree pigment and application of the extract
An extraction method and a technology of shamrock flower, which are applied in the field of natural product extraction, can solve the problems of uneven particle size, easy generation of dust, large environmental pollution, etc., and achieve the effects of natural coloring, nourishing the scalp, and convenient use.
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Embodiment 1
[0041] Crush the dried henna to 50 mesh, add it to the acetic acid-sodium acetate buffer solution with a pH value of 6.0, pasteurize it at 80°C, cool it down to 45°C, and add the activation treated liquid The enzyme activity value of 0.03% cellulase by weight is 6000U / g, and the enzyme activity value of 0.02% pectinase by weight of feed solution is 1000U / g. After 12 hours of enzymolysis, add acetic acid solution for enzyme inactivation treatment. The cell tissue fluid of henna was obtained by suction filtration;
[0042] Add 35% ethanol solution to the cell tissue fluid of Henna in a mass ratio of 3:2, keep the temperature at 35°C, turn on the microwave transmitter, and emit electromagnetic waves with a frequency of 150MHz, continuously penetrating the glandular system, so that the water phase continuously flows into the cells The internal osmosis and the external energy continuously transmitted by electromagnetic waves cause the internal pressure of the cells to increase rapi...
Embodiment 2
[0045]Grind the dried henna to 50 mesh, add it to the acetic acid-sodium acetate buffer solution with a pH value of 7.0, pasteurize it at 80°C, cool it down to 55°C, add the activation treated liquid The enzyme activity value of 0.01% cellulase by weight is 10000U / g, and the enzyme activity value of 0.04% pectinase by weight of feed solution is 5000U / g. After 6 hours of enzymolysis, add acetic acid solution for enzyme inactivation treatment. The cell tissue fluid of henna was obtained by suction filtration;
[0046] Add 50% ethanol solution to the cell tissue fluid of Henna in a mass ratio of 3:1, keep the temperature at 30°C, turn on the microwave transmitter, and emit electromagnetic waves with a frequency of 280MHz, constantly penetrating the glandular system, so that the water phase continuously flows into the cells The internal osmosis and the external energy continuously transmitted by electromagnetic waves cause the internal pressure of the cells to increase rapidly and...
Embodiment 3
[0049] Crush the dried henna to 50 mesh, add it to the acetic acid-sodium acetate buffer solution with a pH value of 4.0, pasteurize it at 80°C, cool it down to 40°C, and add the activation treated liquid The enzyme activity value of 0.04% cellulase by weight is 1000U / g, and the enzyme activity value of 0.01% pectinase by weight of feed solution is 9000U / g. After 10 hours of enzymolysis, add acetic acid solution for enzyme inactivation treatment. The cell tissue fluid of henna was obtained by suction filtration;
[0050] Add 20% ethanol solution to the cell tissue fluid of Henna in a mass ratio of 1:1, keep the temperature at 20°C, turn on the microwave transmitter, and emit electromagnetic waves with a frequency of 50MHz, continuously penetrating the glandular system, so that the water phase continuously flows into the cells The internal osmosis and the external energy continuously transmitted by electromagnetic waves cause the internal pressure of the cells to increase rapid...
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