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A kind of rhodobacter strain and its bacterial agent and its preparation and application

A technology of Rhodobacter genus and strains, applied in the fields of application, bacteria, agricultural machinery and tools, etc., can solve the environmental risks and toxicity of chemical water-retaining agents, achieve good sand-fixing and water-retaining effects, slow down volatilization and infiltration speed, and apply wide range of effects

Inactive Publication Date: 2017-05-17
北京大学包头创新研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The monomers used in synthetic water retaining agents such as acrylamide have certain toxicity, so the use of chemical water retaining agents has potential environmental risks

Method used

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  • A kind of rhodobacter strain and its bacterial agent and its preparation and application
  • A kind of rhodobacter strain and its bacterial agent and its preparation and application
  • A kind of rhodobacter strain and its bacterial agent and its preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, the acquisition and identification of bacterial strain XL-39

[0043] 1. Obtainment of strain XL-39

[0044] Take 500mL of oilfield production fluid and filter it with a 0.22 μm sterile microporous filter membrane, wash the filter membrane with 2mL sterile normal saline, and dilute it in an inorganic salt medium containing 1% petroleum (NaCl 1.0g / L, NH 4 NO 3 1.0g / L, K 2 HPO 4 1.5g / L, KH 2 PO 4 0.5g / L, MgSO 4 7H 2 (O 0.2g / L, others are water, pH7.0~7.2) spread on the plate, cultured at 25°C for 7 days, pick single colony, streak and purify, then save.

[0045] 2. Identification of strain XL-39

[0046] Morphological characteristics: the bacterial body is rod-shaped; the strain grows on LB solid medium plate for 3 days to form red colonies with moist surface.

[0047] Physiological and biochemical characteristics: Negative Gram staining; motile, polar flagella. L-arginine, urea, escin citrate iron, L-arabinose, dextrose mannitol, D-maltose, potass...

Embodiment 2

[0052] Utilize above-mentioned Rhodobacter sp.XL-39 to produce microbial bacterial agent

[0053] 1. Activate the bacterial strain XL-39 (original species) on the LB solid medium plate, and inoculate it on the inclined surface of the test tube for later use.

[0054] 2. Inoculate the test tube seed in a 1-liter shaker flask (containing 200ml broth medium: beef extract 3.0g / L, peptone 10.0g / L, NaCl 5g / L, the rest is water, pH value 7.0-7.2), constant temperature Shake culture to logarithmic phase, ready to inoculate seed pots.

[0055] 3. Prepare fermentation medium (in terms of mass percentage, glucose 0.8%, (NH 4 ) 2 SO 4 1%, K 2 HPO 4 0.2%, MgSO 4 0.05%, NaCl 0.01%, CaCO 3 0.3%, yeast powder 0.02%, pH value 7.2-7.5), 400L fermentation medium was added into 500 liters of seed tank, 121 ℃ autoclaved and heat sterilized, after cooling to 33 ℃, shake the strain of shake flask by 10% (vol. Min content) inoculum amount was inoculated into the seed tank, cultivated to the l...

Embodiment 3

[0057] 5. After the fermentation is completed, the culture solution is taken out of the tank and directly packed into liquid dosage forms with plastic packaging barrels or packaging bottles. Embodiment 3, the water retention of bacterial strain XL-39 in sandy loam

[0058] The soil treatment method is to dry the sandy loam soil taken from the river, weigh 500g soil with a balance and add it to three small pots (every pot is 500g), add 100mL water and 100mL LB liquid medium (yeast powder 5.0 g) to the three pots respectively. g / L, peptone 10g / L, NaCl 10g / L, and others are water) or the bacterial strain culture solution obtained in 100mL above Example 2. The method of weighing the added water (LB liquid medium or strain culture solution) is as follows: take 1mL of LB liquid medium or strain culture solution after drying, weigh the weight, and calculate the water weight. For the above three small pots, weigh the weight of each treatment soil after treating the soil with water or...

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Abstract

The invention discloses a strain of Rhodobacter XL-39 (Rhodobacter sp. XL-39) and a bacterial agent, preparation and application thereof. In the bacterial agent provided by the invention, the active ingredient is Rhodobacter sp. XL-39. The strain and the inoculant have a wide application range, and the water retention rate of the sandy soil can be improved, which can reach more than 15%. The microbial inoculum of the invention has the advantages of low production and use cost and convenience. The invention successfully solves the problems of large water volatilization in arid and semi-arid areas and difficult desertification soil treatment, reduces the workload in the process of ecological reconstruction, and has important significance for protecting the ecological environment and protecting the health of organisms and human bodies.

Description

technical field [0001] The invention relates to a strain of Rhodobacter genus and its bacterial agent as well as its preparation and application. Background technique [0002] my country's water resources are scarce, and the distribution of time and space is uneven. Many areas are seriously short of water. The shortage of water sources has brought serious environmental problems: desertification and salinization of the land have seriously restricted the development of agriculture, animal husbandry, industry and mining. Especially in the arid and semi-arid areas of Northwest China, due to the huge evaporation rate and poor soil water holding capacity, the soil in this area is in a vicious situation of large evaporation-low soil water-few plants-poor soil water holding capacity This is one of the main reasons for the continuous deterioration of the environment and the serious desertification in Northwest China. Rapidly increasing the water-holding capacity of the soil is of gr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K17/00C12R1/01
CPCC09K17/00C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 孙纪全徐莲刘敏吴晓磊
Owner 北京大学包头创新研究院
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