Conjugated polymer particles and methods for their preparation

A polymer particle and conjugation technology, applied in the field of conjugated polymer particles and its preparation, can solve the problems of high signal-to-noise ratio, low precision, low signal, etc.
CN104302783BActive Publication Date: 2018-02-09LIFE TECH CORP
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
LIFE TECH CORP
Publication Date
2018-02-09

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Abstract

The invention discloses a method of conjugating a substrate comprising exchanging a counterion bound to a biomolecule with a lipophilic counterion to form a biomolecular complex, dispersing the biomolecular complex in a non-aqueous solvent, and The biomolecular complex is coupled to the substrate in the presence of an aqueous solvent. The substrate or solid support is a polymer particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counterion is tetrabutylammonium ion, and the non-aqueous solvent is N-methylpyrrolidone (NMP). Oligonucleotide-coated particles are used in nucleic acid isolation or nucleic acid sequencing reactions.
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Description

[0001] This patent application claims the benefit of US Provisional Application No. 61 / 597,064, filed February 9, 2012, which is hereby incorporated by reference in its entirety. technical field

[0002] The present disclosure relates generally to methods of conjugating substrates and substrates formed by such methods. Background technique

[0003] Conjugation of polynucleotides to substrates has become the focus of various industries. Substrates including conjugated polynucleotides are useful in separation techniques, genetic marker detection and sequencing.

[0004] For example, substrates conjugated to polynucleotide probes can be used to capture genetic markers for detection. Exemplary genetic markers can be associated with disease variants within genes, disease-causing bacteria or viruses, or alleles that can be used for identification in humans. Substrates comprising conjugated probes complementary to genetic markers can capture such genetic markers, and a variety of...

Claims

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