A kind of preparation method of 1-1 type human plasma haptoglobin extract

A technology of binding haptoglobin and human plasma, applied in the field of protein separation and purification technology, it can solve the problems of protein structure, functional group damage, inability to extract haptoglobin, limited application scope, etc., to reduce the degradation of haptoglobin and increase the content , the effect of protecting structure and function
CN104327178BActive Publication Date: 2017-12-22INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF FIELD OPERATION SURGERY NO 3 MILITARY MEDICL UNIV PLA
Publication Date
2017-12-22

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Abstract

The invention discloses a preparation method of a human plasma haptoglobin 1-1 extracting solution, which comprises the following step: sequentially carrying out ammonium sulfate fractional precipitation and polyethyleneglycol precipitation on haptoglobin 1-1-containing human plasma centrifugal supernatant preserved at low temperature so as to extract the human plasma haptoglobin 1-1 for fine purification. The purity of the human plasma haptoglobin 1-1 in the extracting solution prepared by the method is greater than 50%; and the method has the advantages of low-temperature operation and mild process, and is very suitable to be used as a crude extraction method for large-scale purification of haptoglobin 1-1 from human plasma.
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Description

technical field

[0001] The invention belongs to the technical field of protein separation and purification technology, and in particular relates to a preparation method of a 1-1 type human plasma haptoglobin extract in the protein separation and purification technology. Background technique

[0002] Haptoglobin (Hp), also known as haptoglobin, is an acidic glycoprotein widely present in mammalian blood and other body fluids. It is mainly synthesized and degraded by the liver, and its content is regulated by lipopolysaccharide, cytokines, hormones, etc. An acute phase response protein. The haptoglobin molecule is composed of a and β subunits, and in most mammals (aβ) 2 There are three main subtypes of human haptoglobin, 1-1, 2-1, and 2-2, which are mainly caused by the diversity of a subunit. Human haptoglobin a subunit is encoded by alleles Hp1, Hp2, Hp1 encodes a 1 Subunit (molecular weight about 9.1kDa), Hp2 encodes a 2 subunit (molecular weight about 16kDa). Human ha...

Claims

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