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Application of strychnine and its preparation in preparation of medicine for treating breast cancer

A technology of strychnine and total alkaloids of strychnine, which is applied in the direction of drug combination, antineoplastic drugs, and pharmaceutical formulations, can solve the problems of patients with weak constitutions and lost opportunities for treatment, etc., to reduce toxic effects and improve safety , reducing the effect of stimulation

Active Publication Date: 2020-07-14
BEIJING INCREASEPHARM CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although its treatment has entered the stage of comprehensive treatment, and many new drugs such as paclitaxel, taxotere, letrava, and herceptin have further improved the curative effect of advanced breast cancer, there are still some patients who suffer from chemotherapy due to their weak constitution. The ability and the opportunity to lose treatment due to drug resistance and other reasons during treatment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Preparation method of strychnine total alkali vesicle gel:

[0018] Take 0.5 g of strychnose total alkali, 2.5 g of Span 60, and 0.25 g of cholesterol, dissolve in absolute ethanol, remove the solvent by rotary evaporation (60°C), add to ammonium sulfate buffer with pH 6.8, and stir at 20°C until ethanol After evaporating, sonicating for 30 minutes, standing and cooling at room temperature, the vesicle suspension of total alkaloids of strychnine was obtained.

[0019] Take 0.1g of HPMC (K-6000), 1g of glycerol, 0.5g of potassium sorbate, add 100ml of purified water to prepare a blank gel matrix, and mix the vesicle suspension with the blank gel matrix to obtain strychnose Total alkali vesicle gel.

Embodiment 2

[0021] The preparation method of strychnine total alkali microemulsion gel:

[0022] Weigh 4g of the total alkali of Strychnos, 16g of ethyl oleate, 35g of the polyoxyethylene castor oil EL-35, 15g of PEG400, and 30g of the water for injection, and stir them to obtain the total alkali of Strychnine microemulsion.

[0023] Dissolve 0.2g of propyl paraben in 10g of propylene glycol, weigh 1g of carbomer 980 and add appropriate amount of water to swell, add sodium hydroxide to adjust the pH to about 7, and 5g of glycerol, 3g of azone, and propylene glycol with preservatives Mix uniformly to obtain a gel matrix, add 30 g of total alkaloids of strychnose and 50 g of water, and stir evenly to obtain a total alkali of strychnose microemulsion gel.

Embodiment 3

[0025] The inhibitory effect of strychnine total alkali on breast cancer:

[0026] In the in vivo experiment, the cultured human breast cancer cell line ZR-75-30 was diluted in the culture medium to make the cell density 2.5×107 cells / ml, and each nude mouse was subcutaneously inoculated with 0.2ml on the right second nipple After inoculation, place it in a breeding room without special pathogens under the fat pad, and regularly observe and record the tumor growth. When the tumor volume is nearly 100mm 3 Time (use vernier caliper to measure the volume, calculation formula: V=a×b 2 / 2, a is the long diameter of the tumor, b is the short diameter of the tumor), record the initial tumor volume and initial body weight, and group the drugs into groups. The in vivo experiment is divided into 3 groups, 6 mice / group, totaling 18 mice.

[0027] Negative control group: 0.5% CMC-Na saline group.

[0028] Administration group 1: Strychnoma vulgaris total alkali vesicle gel group (the preparation...

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Abstract

The invention discloses semen strychni total alkali and new application of a preparation of the semen strychni total alkali, in particular to application of semen strychni total alkali vesica gel and semen strychni total alkali microemlusion-based gel to preparing medicines for treating the breast cancer. Animal in-vivo anticancer pharmacology experiments show that the semen strychni total alkali has the inhibiting effect on ZR-75-30 nude mouse transplantation tumor growth; and the semen strychni total alkali can be used for preparing the medicines for treating the breast cancer.

Description

Technical field [0001] The invention relates to a new use of strychnine, in particular to the application of strychnine total alkaloids and preparations thereof in the preparation of drugs for treating breast cancer. Background technique [0002] Breast cancer is a serious disease that threatens women's physical and mental health. It ranks first among female malignant tumors, and it has been on the rise in recent years. Although its treatment has entered the stage of comprehensive treatment, and many new drugs such as paclitaxel, taxotere, lettrova, Herceptin, etc. have further improved the efficacy of advanced breast cancer, some patients still suffer from chemotherapy due to their weak physique. The ability of the disease and the emergence of drug resistance during the treatment process and the loss of treatment opportunity. [0003] At present, in anti-tumor research, inducing cancer cell apoptosis is an important way. Promoting tumor cell apoptosis can achieve tumor shrinkage ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/475A61K36/56A61K9/06A61P35/00
CPCA61K9/06A61K36/56
Inventor 张保献
Owner BEIJING INCREASEPHARM CORP LTD