Screening method for efficiently obtaining hexaploid echinacea

A screening method and technology of Echinacea, applied in the fields of crop biotechnology and genetic breeding, can solve problems such as low screening efficiency, achieve good economic and social benefits, good practical application value, and broad application prospects.

Active Publication Date: 2015-02-25
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to overcome the defect of low screening efficiency of succe

Method used

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  • Screening method for efficiently obtaining hexaploid echinacea
  • Screening method for efficiently obtaining hexaploid echinacea
  • Screening method for efficiently obtaining hexaploid echinacea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 The Effect of Colchicine on the Survival Status of Different Types of Explants

[0019] The diploid seeds of Echinacea purpurea used in the present invention are purchased from the Plantation Products horticultural company of Norton, Massachusetts, U.S.; Tetraploid carries out colchicine treatment to diploid Echinacea purpurea through applicant's existing method and its chromosome doubling is obtained (Nilanthi, 2009); triploids were obtained by crossing diploids and tetraploids and cultivating seeds.

[0020] The leaves, petioles and root explants of the obtained triploid Echinacea purpurea were treated with 120 mg / L colchicine for 18 days, and then transferred to plants containing BA 0.4 mg / L, NAA 0.01 mg / L, and sucrose 30 g / L. , cultured on agar 4.5 g / L MS regeneration medium, and counted the survival rate of explants after 22 days. It can be seen from Table 1 that the culture responses of different types of triploid explants to the treatment of 120 mg / L...

Embodiment 2

[0022] Example 2 The effect of root explant selection position on induction of adventitious buds after colchicine treatment

[0023] For the same triploid line, after 30 days of rooting, select roots with similar states, and intercept different positions (near the root tip, middle, and far from the root tip). BA 0.4 mg / L, NAA 0.01 mg / L, sucrose 30 g / L, agar 4.5 g / L MS medium, at 23-27°C, cool white light intensity 600-1300 lx, photoperiod 12 h / d After culturing for 25 days under the same conditions, the adventitious buds were induced to regenerate on MS medium containing 0.5 mg / L BA, and the induction rate of adventitious buds was studied. According to Table 2, as long as the roots are rooted at the same time, and the selected roots are close to each other, no matter whether the root section close to the root tip is used as the explant or the root section far away from the root tip is used as the explant, the After the doubling of narcissine, there was no significant differ...

Embodiment 3

[0025] Example 3 The relationship between the time span from induction of adventitious bud regeneration to root tip chromosome inspection and its hexaploid acquisition rate

[0026] After the root explants treated with 120 mg / L colchicine for 18 days were transferred to MS medium containing 0.5 mg / L BA to induce adventitious shoots, record the transfer date a; when the regenerated shoots grow to When the leaves are 2 cm high, transfer them to the rooting medium (MS medium containing NAA 0.01 mg / L, sucrose 30 g / L, agar 4.5 g / L), and put them under the condition of 23-27℃, cool white light intensity 600~ Cultured under the conditions of 1300 lx and a light cycle of 12 h / d to induce adventitious roots, when the adventitious roots grow to 2 cm, record the date b. The time span from the induction of adventitious bud regeneration to root tip chromosome inspection is the growth time, recorded as b-a days.

[0027] It can be seen from Table 3 that the results show that the growth t...

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Abstract

The invention belongs to the field of crop biological technology and inheritance and breeding technology, and particularly relates to a screening method for efficiently obtaining hexaploid echinacea. The screening method comprises the following steps: inducing root explants of triploid echinacea treated by chromosome doubling to regenerate adventitious buds; after the adventitious buds grow to be 2cm high, inducing to root; and selecting the adventitious buds with the root being 2cm 150-210 days after the induction of the adventitious buds to carry out ploidy identification, so as to obtain the echinacea which is doubled into a hexaploid from a triploid at a high success rate. According to the method provided by the invention, the success rate of the screened hexaploid is up to 53.33% and a reliable technical means for screening the multiploid echinacea is provided; an important technical support is provided for the breeding of the multiploid echinacea and the application prospect is wide; and the screening method has a good actual application value, and better economic benefits and social benefits can be generated.

Description

technical field [0001] The invention relates to the fields of crop biotechnology and genetic breeding, and more particularly relates to a screening method for efficiently obtaining hexaploid Echinacea purpurea. Background technique [0002] Echinacea ( Echinacea purpurea ) is a perennial herb of Compositae, a wild flower native to America, which has been widely used in nutritional supplements and health food because of its good immune regulation effect. Since the active ingredients of Echinacea purpurea mainly exist in the roots, in order to increase the yield of roots, further improve the quality, increase the content of active ingredients, and enhance its disease resistance, the research on ploidy breeding around it has developed rapidly. Explants successfully cultivated tetraploid, octaploid research, but the induction success rate is low; in the prior art, the efficiency of screening successfully doubled polyploid Echinacea purpurea is low. Contents of the invention ...

Claims

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Application Information

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IPC IPC(8): A01H1/08
Inventor 杨跃生陈晓鹭吴鸿李栋梁刘颖
Owner SOUTH CHINA AGRI UNIV
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