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Method for producing AM fungal inoculant

A production method and technology of fungal inoculum, applied in the production field of AM fungal inoculum, can solve the problems of low quality of inoculum, low infection rate, and failure to overcome, and achieve good inoculation effect and high infection rate

Inactive Publication Date: 2015-02-25
NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The above beneficial effects of AM fungi on plants are limited to small-scale scientific experiments, and the large-scale application in production has not been reported. The main reason is that the production technology of AM fungi is not mature enough, and the quality of the produced bacteria is not high. , low infection rate
[0005] There is currently no method to overcome the above drawbacks

Method used

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  • Method for producing AM fungal inoculant
  • Method for producing AM fungal inoculant
  • Method for producing AM fungal inoculant

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Embodiment Construction

[0036] A kind of production method of AM fungal inoculant, the method comprises the steps:

[0037] a. Pregermination of corn: select corn seeds for cultivation with full grains, no damage, and no insect bites, first sterilize with 75% alcohol solution for 30 seconds, then rinse with sterile water for 5 times, and place them in an incubator at a temperature of 28°C , dark germination;

[0038] b. Inoculation: Fill each flower pot with 1 / 3 of the culture medium first, then sprinkle 0.5-1.0cm of inoculant, and spread 1 / 3 of the culture medium, sprinkle 20-25 red clover seeds and 3 seeds in each pot For the corn that has been germinated, the pot is filled with sterilized river sand substrate and watered;

[0039]c. Seedling stage management: as shown in Table 1: configure Hoagland nutrient solution, use method: within 30 days of the seedling stage, pour 1 / 2 strength nutrient solution once a week, all nutrient elements are halved, 100mL / pot; After that, the full nutrient solutio...

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Abstract

The invention relates to a method for producing a fungal inoculant, and particularly relates to a method for producing an AM fungal inoculant. The method is characterized by comprising the following steps: carrying out pregermination, inoculation, seedling-stage management and inoculant quality analysis on a corn; and 4 months after inoculation, collecting the root system of a host plant, and inquiring the dip dyeing station and dip dyeing rate of AM fungi by adopting Phillips and Hayman dyeing methods for the quality evaluation of an inoculant. The method disclosed by the invention has the advantages of uniqueness, high inoculant quality and high dip dyeing rate.

Description

technical field [0001] The invention relates to a production method of fungal inoculum, in particular to a production method of AM fungal inoculum. Background technique [0002] Mycorrhizae (Mycorrhizae) are symbionts formed by mycorrhizal fungi and plant roots. More than 93% of plants in nature can form mycorrhizae. AM fungi are also called arbuscular mycorrhizal fungi (Arbuscular mycorrhiza Fungi, referred to as AMF). Previous studies believe that AM fungi can promote the absorption of mineral nutrients, especially phosphorus and trace elements, enhance the ability of plants to resist nutrient stress, reduce the use and loss of nitrogen and phosphorus fertilizers, and avoid eutrophication of water bodies. At the same time, its hyphae can form mycelial bridges, transfer nutrients between different plants, and become an important channel for the nutrient cycle of the ecosystem. AM fungus can improve the stress resistance of plants: it can improve the water status of plant...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 王亚军安巍罗青唐明曹有龙石志刚赵建华张海涵刘洪光张曦燕尹跃
Owner NINGXIA ACADEMY OF AGRI & FORESTRY SCI
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