Method for Concentrating and Separating Catalin from Fermented Broth of Endophytic Fungus of Catalpa and Its Application

Abstract

The invention discloses a method for condensation and separation of catalpa bungei essence Qiulingsu in catalpa bungei endophytic fungus broth and a use thereof. The method comprises the following steps of 1, adjusting a pH value of a catalpa bungei endophytic fungus broth to 4-10, 2, pre-cooling the catalpa bungei endophytic fungus broth subjected to pH adjustment to a pre-cooling temperature of 0-4 DEG C, 3, slowly cooling the catalpa bungei endophytic fungus broth in the case of crystal nucleus existence until the catalpa bungei endophytic fungus broth freezes, and 4, carrying out slow cooling freezing until the rest of the liquid broth is concentrated to a preset volume, stopping cooling and collecting the unfrozen liquid which is catalpa bungei essence Qiulingsu concentrate. The catalpa bungei endophytic fungus broth is concentrated to form the broth with 1 / 10 of the original volume and a catalpa bungei essence Qiulingsu yield is more than 90%. Compared with the prior art, the method effectively guarantees stabilization of catalpa bungei essence Qiulingsu in the raw material, and a high condensation yield, is free of any organic solvent and guarantees concentrate quality.

Description

technical field

[0001] The invention relates to the field of biochemistry, in particular to a method for concentrating and separating catalpaline from the fermented liquid of endophytic fungi of catalpa and its application. Background technique

[0002] Endophytic fungi are those fungi that live inside plant tissues and do not cause obvious plant diseases. Endophytic fungi can produce the same or similar bioactive substances as the host plant, which has great potential value and can be a source of new anticancer drugs. Our research group has isolated and extracted an endophytic fungus (CCTCC M 208102) with strong anti-cancer activity from Juglans chinensis, and initially identified the fungus as Deuteromycotina, Hyphomycetes, Polychospora, Wood Mold belongs to the genus, and its fermented extract has a strong inhibitory effect on liver cancer cells. This is a brand-new strain unique to our laboratory, and its fermentation broth preparation method with anticancer ability ha...