Preparation method of temperature sensitive hydrogels

A sensitive, hydrogel technology, applied in the field of preparation of poly/γ-polyglutamic acid thermosensitive hydrogel, can solve the problems of complicated system purification process and long preparation time, achieve good drug-carrying performance and improve biological phase. Compatibility, good biocompatibility

Active Publication Date: 2015-03-04
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This preparation method takes a long time, the PGA used is chemically syn

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: When the molar ratio of PNIPAM:γ-PGA is 9:2, that is, 0.16g of PNIPAM and 0.04g of γ-PGA are dissolved in 1mL of distilled water, stirred and dissolved, and the air bubbles are removed overnight at 4°C to obtain a colorless and transparent uniform mixed solution , 37 ° C water bath, 7min system from a colorless transparent solution to milky white gel, no dehydration. It is swelled in water at 37°C, and the measured water absorption rate is 746.87%. The LCST was 31° C. through rheological detection and analysis.

Embodiment 2

[0012] Example 2: When the molar ratio of PNIPAM:γ-PGA is 7:2, that is, 0.15g of PNIPAM and 0.05g of γ-PGA are dissolved in 1mL of distilled water, stirred and dissolved, and bubbles are removed overnight at 4°C to obtain a colorless and transparent uniform mixed solution , 37 ° C water bath, 6min system from a colorless transparent solution to milky white gel, no dehydration. It is swelled in water at 37°C, and the measured water absorption rate is 891.89%. After rheological analysis, the LCST was 29°C.

Embodiment 3

[0013] Example 3: When PNIPAM:γ-PGA molar ratio is 7:4, 0.12g of PNIPAM and 0.08g of γ-PGA are dissolved in 1mL of distilled water, stirred and dissolved, and bubbles are removed overnight at 4°C to obtain a colorless and transparent uniform mixed solution. In a water bath at 37°C, the system changed from a colorless transparent solution to a milky white gel within 3 minutes without dehydration. It is swelled in water at 37°C, and the measured water absorption rate is 1289.86%. After rheological detection and analysis, the LCST was 28°C.

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Abstract

The invention provides a preparation method of temperature sensitive hydrogels. The preparation method comprises the following steps: dissolving poly(N-isopropylacrylamide) (PNIPAM) and gamma-polyglutamic acid (gamma-PGA) in distilled water, dissolving by stirring at a room temperature for 1-3 hours, standing overnight at 4 DEG C, and removing bubbles, thus obtaining a uniform aqueous solution; and carrying out water bath at 37 DEG C for 1-8 minutes until the system turns to a milk white gel from a colorless and transparent solution without being dehydrated, wherein the feeding molar ratio of PNIPAM to gamma-PGA ranges from 9: 2 to 7: 6, and the total concentration of the solution is 20%(w/v). The hydrogels prepared by use of the preparation method have temperature sensitivity and also has excellent water absorptivity and water retentivity; the preparation method is simple and time-saving; the preparation method is high in gel forming speed and can be used for preparing temperature sensitive hydrogels different in water absorbency and lower critical solution temperature (LCST) by controlling the feed ratio of PNIPAM to gamma-PGA, and thus is wide in application range. The temperature sensitive hydrogels prepared by use of the preparation method can be applied to drug carriers, wound dressings and the like due to excellent biocompatibility and drug carrying property.

Description

Technical field: [0001] The present invention relates to a kind of preparation method of temperature-sensitive hydrogel (abbreviated as temperature-sensitive hydrogel), specifically the method of compounding by heating poly(N-isopropylacrylamide) and γ-polyglutamic acid in aqueous solution Preparation of poly(N-isopropylacrylamide) / γ-polyglutamic acid thermosensitive hydrogel. Background technique: [0002] Poly(N-isopropylacrylamide), PNIPAM for short, has attracted widespread attention, especially in the biomedical field, for its temperature sensitivity. Because PNIPAM has both hydrophilic and hydrophobic groups in its side chains, the linear PNIPAM aqueous solution exhibits temperature sensitivity. At room temperature, linear PNIPAM forms a homogeneous solution in water. When the temperature rises to 32°C, the system changes from a flowable sol to an immobile gel. Therefore, the PNIPAM temperature-sensitive system can be used to load drugs for the biomedical field. The...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L33/24C08L77/04A61K47/30A61L15/22
Inventor 王淑芳庄华红杨希马祎娜宋存江
Owner NANKAI UNIV
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