Preparation method of temperature sensitive hydrogels

Abstract

The invention provides a preparation method of temperature sensitive hydrogels. The preparation method comprises the following steps: dissolving poly(N-isopropylacrylamide) (PNIPAM) and gamma-polyglutamic acid (gamma-PGA) in distilled water, dissolving by stirring at a room temperature for 1-3 hours, standing overnight at 4 DEG C, and removing bubbles, thus obtaining a uniform aqueous solution; and carrying out water bath at 37 DEG C for 1-8 minutes until the system turns to a milk white gel from a colorless and transparent solution without being dehydrated, wherein the feeding molar ratio of PNIPAM to gamma-PGA ranges from 9: 2 to 7: 6, and the total concentration of the solution is 20%(w / v). The hydrogels prepared by use of the preparation method have temperature sensitivity and also has excellent water absorptivity and water retentivity; the preparation method is simple and time-saving; the preparation method is high in gel forming speed and can be used for preparing temperature sensitive hydrogels different in water absorbency and lower critical solution temperature (LCST) by controlling the feed ratio of PNIPAM to gamma-PGA, and thus is wide in application range. The temperature sensitive hydrogels prepared by use of the preparation method can be applied to drug carriers, wound dressings and the like due to excellent biocompatibility and drug carrying property.

Description

Technical field: [0001] The present invention relates to a kind of preparation method of temperature-sensitive hydrogel (abbreviated as temperature-sensitive hydrogel), specifically the method of compounding by heating poly(N-isopropylacrylamide) and γ-polyglutamic acid in aqueous solution Preparation of poly(N-isopropylacrylamide) / γ-polyglutamic acid thermosensitive hydrogel. Background technique: [0002] Poly(N-isopropylacrylamide), PNIPAM for short, has attracted widespread attention, especially in the biomedical field, for its temperature sensitivity. Because PNIPAM has both hydrophilic and hydrophobic groups in its side chains, the linear PNIPAM aqueous solution exhibits temperature sensitivity. At room temperature, linear PNIPAM forms a homogeneous solution in water. When the temperature rises to 32°C, the system changes from a flowable sol to an immobile gel. Therefore, the PNIPAM temperature-sensitive system can be used to load drugs for the biomedical field. The...

AI Technical Summary

Problems solved by technology

This preparation method takes a long time, the PGA used is chemically synthesized, and is α-PGA, and the system purification process is cumbersome