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Anti-cancer composition with synergistic effect

A synergistic and composition technology, which can be used in medical preparations containing active ingredients, drug combinations, anti-tumor drugs, etc. It can solve the hidden dangers of recurrence and metastasis, neglect the removal of tumor proliferation pools, and it is difficult to achieve radical cure. , to solve the problem of tumor drug resistance, reduce the risk of treatment, and inhibit growth

Inactive Publication Date: 2015-03-25
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although current cancer chemotherapy can successfully reduce the burden of many solid tumors and eliminate rapidly proliferating cells, it is difficult to achieve a radical cure. The root cause of its failure may be ignoring the removal of tumor proliferation pools, laying hidden dangers for future recurrence and metastasis

Method used

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  • Anti-cancer composition with synergistic effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Inhibitory effect of SN and DA alone on the proliferation of breast cancer cells .

[0029] Cells in logarithmic growth phase were taken, digested with 0.25% trypsin-0.53mmol / L EDTA solution, and counted. MCF-7 (human breast cancer cells), MCF-7 / Adr (MCF-7 cells resistant to doxorubicin), and breast cancer stem cells were divided into 1 × 10 4 cells / well were seeded in 96-well cell culture plates. After culturing for 24 hours, administration was carried out. The final concentrations of the SN single-drug administration group were 0, 0.01, 0.1, 1, 2, 5, 10, and 100 μM; the final concentrations of the DA single-drug administration group were 0, 0.01, 0.1, 1, 10, 20, 50, 100 μM, set 6 parallels in each group, and continue to incubate for 48 hours. When investigating the combined administration, for four parallel 96-well plates, the concentration of DA was set to 0, 2, 20, 100 μM in turn, while in each 96-well plate, the concentration of SN was set to 0, 1, 2, 5, 10 μM...

Embodiment 2

[0035] Inhibitory Effects of SN and DA on the Proliferation of MCF-7 / Adr Cells and Tumor Stem Cells .

[0036] The inhibitory effect of different concentrations of SN and different concentrations of DA on cell proliferation was investigated by SRB method, and the size of the combination index (CI) was analyzed. The synergy index was calculated by the method reported in the literature, and the theoretical absorbance of the combined administration group was calculated according to formula 1-3, and the ratio of the measured absorbance to the theoretical value was the synergy index (formula 1-4). CI values ​​less than, equal to and greater than 1 indicate that SN and DA have synergistic, additive and antagonistic effects, respectively.

[0037]

[0038] The results are displayed (see figure 2 ), regardless of MCF-7 / Adr cells or tumor stem cells, the combination of DA significantly enhanced the inhibitory effect of SN on cancer cells, and it was enhanced with the increase of...

Embodiment 3

[0040] Inhibitory Effects of Combination of SN and DA on Tumor in Drug-resistant Xenograft Model of Breast Cancer in Vivo (Single Dose)

[0041] BALB / c nude mice were bred for one week and then inoculated with MCF-7 / Adr cells. MCF-7 / Adr cells in the logarithmic growth phase were digested with 0.25% trypsin-0.53mmol / L EDTA solution to prepare a single cell suspension, centrifuged at 1,000rpm for 5min, the cells were washed twice with PBS, and the cells were adjusted with PBS Density is 1×10 7 pieces / ml. Subcutaneously inject 0.2ml of cell suspension (approximately 2×10 6 cells). The long diameter of the tumor was measured with a vernier caliper (D max ) and short diameter (D min ), calculate the tumor volume V (V=D max ×D min 2 / 2).

[0042] MCF-7 / Adr cells were orthotopically inoculated into the mammary glands of 12 BALB / c nude mice to obtain a xenografted breast cancer tumor model, when the average tumor volume of the nude mice was about 100mm 3 , were randomly di...

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Abstract

The invention relates to an anti-cancer composition with synergistic effect, specifically a composition comprising an effective amount of at least one VEGF / VEGFR inhibitor and dopamine. The composition provided by the invention can obviously inhibit the growth of tumor cells, substantially reduce the use of anticancer drugs, and reduce the treatment risk and cost, and has broad application prospects.

Description

technical field [0001] The invention relates to an anticancer composition with synergistic effect, its preparation method and application. Background technique [0002] Cancer, also known as malignant tumor, is a disease caused by the abnormality of the mechanism controlling cell growth and proliferation. In addition to growing out of control, cancer cells can locally invade surrounding tissues and even metastasize to other parts of the body. At present, the traditional methods of treating cancer include radiotherapy or chemotherapy. For some local solid tumors, electrotherapy can be used. These traditional methods have certain curative effects, but their side effects are quite large. big pain. In tumor diseases, solid tumors account for the vast majority, and the occurrence, development, recurrence and metastasis of solid tumors all depend on the formation of tumor angiogenesis. Tumor angiogenesis is essential for the growth and metastasis of solid tumors. Tumor starvat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K38/18A61K31/137A61P35/00
Inventor 周田彦李良卢炜王思媛冀希炜马元亨
Owner PEKING UNIV
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