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A method for the detection of low-concentration mercury ions based on a DNA-modified SERS substrate

A DNA molecule, mercury ion technology, applied in the preparation of test samples, Raman scattering, material excitation analysis, etc., can solve the problem of unable to generate Raman signal, unable to directly measure metal ions, etc., to achieve strong SERS enhancement effect, Good uniformity of morphology and particle size, and the effect of reducing interference

Active Publication Date: 2017-08-25
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, metal ions, including mercury ions, do not generate Raman signals by themselves, so this spectroscopic technique cannot directly measure metal ions

Method used

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  • A method for the detection of low-concentration mercury ions based on a DNA-modified SERS substrate
  • A method for the detection of low-concentration mercury ions based on a DNA-modified SERS substrate
  • A method for the detection of low-concentration mercury ions based on a DNA-modified SERS substrate

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Embodiment Construction

[0022] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0023] see figure 2 , a method for detecting low-concentration mercury ions based on a DNA-modified SERS substrate of the present invention, comprising the following steps:

[0024] (1) Uniform gold-coated silica nanoparticles were prepared by a step-by-step method, and the core-shell nanoparticles were immobilized on a quartz glass plate using a coupling agent to construct a SERS substrate.

[0025] Specifically include:

[0026] 1a: First prepare silica nanoparticles.

[0027] 1b: Synthesizing Au gold seeds, specifically synthesizing 1-3nm Au gold seeds.

[0028] 1c: Preparation of SiO 2 @Au core-shell nano...

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Abstract

The invention discloses a method for detecting low-concentration mercury ions based on a DNA-modified SERS substrate, adopting a step-by-step method to prepare uniform gold-coated silica nanoparticles, and using a coupling agent to fix the core-shell nanoparticle on the quartz glass On-chip construction of the SERS substrate. The single-stranded DNA molecule is modified on the surface of the gold shell layer of gold-coated silica nanoparticles through thiol groups. Soak the prepared DNA aptamer-modified SERS substrate in a solution containing mercury ions, and wash it with deionized water. The SERS signal ratio of G base and A base is used as the spectral standard measurement of DNA on the metal surface, and the quantitative and specific detection of mercury ions is realized by analyzing the characteristic Raman peaks and their ratio changes. Compared with the prior art, the present invention has the following advantages: the method of the present invention has high sensitivity, and the detection limit is about 50nM; and the measurement operation is simple, the cost is low, and real-time and rapid measurement can be realized.

Description

technical field [0001] The invention relates to a method for detecting low-concentration mercury ions based on a DNA-modified SERS substrate. Background technique [0002] Mercury ion is a common pollutant in the water environment, causing serious harm to human health and ecological environment. Mercury ions, especially methylmercury, can accumulate in the human body through the food chain, causing permanent damage to the central nervous system, digestive system and kidneys and causing various chronic diseases. Methylmercury is the water-soluble mercury ion (Hg 2 + ) from methylation. Therefore, the quantitative and specific detection of mercury ions in the water environment is a problem that everyone has been concerned about. The general methods for detecting mercury ions are often not high in sensitivity or specificity, and are inconvenient to operate. Recently, people use Thymine-Hg 2+ -Thymine (T-Hg 2+ The coordination chemistry of -T) has designed some new detect...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65G01N1/28
Inventor 黄青鲁逸林
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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