Anti-SM-RNP antibody kit

A kit and antibody technology, which is applied in the field of anti-SM-RNP antibody kits, can solve problems such as unstable results, unstable antibodies, and unstable positive control antibodies, and achieve low experimental costs, easy access, and high similarity Effect

Inactive Publication Date: 2015-03-25
CHENGDU VERO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by this invention is to provide an anti-SM-RNP antibody test kit to overcome the instability of the positive control antibody of each batch when the existing test kit detects the content of anti-SM-RNP antibody in human serum. problems that lead to unstable results
[0006] The positive control serum in the existing anti-SM-RNP antibody kit comes from the positive control serum of the patient, so that the positive control may be different for each batch of kits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0018] An anti-SM-RNP antibody test kit, the test kit includes a cell carrier for expressing an anti-SM-RNP antibody, a negative control serum, a positive control serum, an enzyme substrate, a concentrated sample diluent, and the positive control serum is patient's Control serum after positive control serum screening. Cell carriers are mammalian cells.

[0019] A preparation method for an anti-SM-RNP antibody kit, comprising the following steps:

[0020] First, the preparation of the cell carrier: After activating the cell carrier expressing the anti-SM-RNP antibody, incubate at 4°C for 5 hours, wash with PBS buffer, centrifuge, discard the supernatant, add a certain concentration of serum protein blocking solution, Incubate for 30 minutes to complete the preparation of the cell carrier;

[0021] Secondly, the preparation of other components: the negative control serum is the serum of healthy people, the positive control serum is the confirmed anti-SM-RNP antibody positive s...

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PUM

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Abstract

The invention discloses an anti-SM-RNP (SM-ribonucleoprotein) antibody kit which comprises a cell carrier used for expressing an anti-SM-RNP antibody, negative control serum, positive control serum, an enzyme substrate and concentrated sample dilution liquid, wherein the positive control serum is control serum after the positive control serum of patients is screened. According to the anti-SM-RNP antibody kit, the collected positive control serum is screened and the positive control serum with a densely distributed numerical value region is selected as the positive control serum in the anti-SM-RNP antibody kit, so that the problem of an instable result caused by an instable positive control antibody in each batch during detection of the SM-RNP antibody content of human serum by an existing kit is solved.

Description

technical field [0001] The invention relates to the field of kits, in particular to an anti-SM-RNP antibody kit. Background technique [0002] Systemic lupus erythematosus (SLE) is a chronic systemic autoimmune disease characterized by the formation of a large number of autoantibodies, leading to multi-system and multi-organ damage. Systemic lupus erythematosus is a common rheumatic disease. According to investigations, the incidence of the disease is about 1 / 1000, and it is more common in women of childbearing age. The ratio of male to female is about 1:7-10. The advantage is not obvious. The main etiology may be related to genetics, environmental factors and estrogen. Studies have reported that age is a major risk factor, especially in young women. The pathogenesis is mainly related to abnormal immune regulation, a large number of autoantibodies, and the formation of immune complexes. At present, the specific pathogenesis is still unclear. [0003] Serum detection of SL...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/531
CPCG01N33/53G01N33/531
Inventor 张志新叶正义何远立杨秦
Owner CHENGDU VERO BIOTECH
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