Immunohistochemical SIMPLE same part marker diagnosis method and kit for pathologic autopsy anaphylactic shock

Abstract

The invention provides an immunohistochemical SIMPLE same part marker diagnosis method and kit for a pathologic autopsy anaphylactic shock. The kit comprises a first mastocyte (MC) antibody, a first IgE antibody, a universal second antibody reagent, an antigen repair reagent, a DAB developing solution, an AEC developing solution, a buffer liquid system and the like, wherein the first antibody is a mouse-anti-human monoclonal MC antibody; and the first IgE antibody is a mouse-anti-human monoclonal IgE antibody. The kit has the characterstics of being low in price, good in specificity, high in sensitivity, high in accuracy, good in stability, simple and general. The kit is mainly applied to identification of pathology and forensic pathology; and an intuitive pathological basis for the anaphylactic shock can be accurately provided.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and relates to an immunohistochemical SIMPLE same-site labeling diagnosis method for anaphylactic shock in pathological autopsy and a kit thereof. Background technique [0002] Anaphylaxis (anaphylaxis, anaphylactic shock) is a type I allergic reaction, which is a strong multi-organ involvement syndrome that occurs in a short period of time through the immune mechanism after certain external antigenic substances enter the sensitized body. [0003] MC and degranulation after MC activation are the core of the occurrence and development of anaphylactic shock. After the MC is activated, the tryptase-rich granules and histamine granules in the MC are released into the blood. The extravasation of tryptase in the MC has been used as one of the important indicators to measure whether there is anaphylactic shock. In addition to the method of detecting the enzyme in serum, the content of tryptas...

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Problems solved by technology

[0004] Anaphylactic shock is one of the most frequent clinical emergencies, accounting for about 10% of forensic disputes every year. However, due to the sudden onset of the disease, only non-specific manifestations such as congestion and edema of tissues and organs at the time of sudden death can be seen during autopsy, and it is difficult to find clear morphological findings. Changes, the forensic medical forensic identification needs to combine the history of allergies and medications of the deceased, and exclude other causes of death to make a diagnosis, which brings great inconvenience to the forensic diagnosis of anaphylactic shock, so finding simple and easy diagnostic indicators has become an urgent solved problem

MC, degranulation MC, IgE, and EOS have played a great role in the mechanism of anaphylactic shock, but the degranulation rate of MC has been rarely reported so far, and IgE and EOS are mostly limited to animal models, and their effects have not yet appeared in humans. Discussion on Joint Determination and Its Significance

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