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Wild jujube stem and leaf extract, as well as preparation method and application thereof

A technology of jujube branches and leaves and extracts, which are used in the preparation of treatment for sedation and hypnosis, jujube branches and leaves extract and preparation thereof, and application fields of antidepressant drugs

Active Publication Date: 2015-04-29
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of pharmacological effects, the sedative, hypnotic and antidepressant effects of Zizyphus spinosus seed are more in-depth, while the reports of anti-insomnia and antidepressant effects of Zizyphus jujube leaves are less

Method used

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  • Wild jujube stem and leaf extract, as well as preparation method and application thereof
  • Wild jujube stem and leaf extract, as well as preparation method and application thereof
  • Wild jujube stem and leaf extract, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] After crushing 100 g of wild jujube branches and leaves, it was heated and refluxed for 3 times with a 20-fold amount of 80% ethanol solution for 1 hour each time. The solvent was recovered under reduced pressure and concentrated to obtain an extract. Then, 10 times the weight of the extract was added with distilled water. Dissolve the extract under the conditions of, and pass the above solution through the D101 macroporous resin chromatography column. The amount of macroporous resin is 10 times the weight of the extract. First elution with water, rinse until the phenol-sulfuric acid test solution has a negative reaction, and the flow rate is 1 Column volume / hour, and then use gradient elution with volume concentration of 10%, 30%, 40%, 60%, 70%, and 90% ethanol solution, flow rate is 1 column volume / hour, and the amount is column 10 times the volume, discard the water and 10-30% ethanol eluent to obtain 40%-60% and 70%-90% ethanol eluent, measured by spectrophotometry in...

Embodiment 2

[0053] After crushing 100g of wild jujube branches and leaves, flash extract twice with 8 times the amount of 30% ethanol solution for 1 min each time, recover the solvent and concentrate to obtain the extract, and then add 5 times the weight of the extract with distilled water under ultrasonic conditions Next, dissolve the extract, and pass the above solution through D101 macroporous resin chromatography column. The amount of macroporous resin is 10 times the weight of the extract. First elution with water, rinse until the phenol-sulfuric acid test solution is negative, and the flow rate is 1 column. Volume / hour, and then use gradient elution with volume concentration of 10%, 30%, 40%, 60%, 70%, and 80% ethanol solution, flow rate is 1 column volume / hour, and the amount is the column volume 10 times, discard the water and 10-30% ethanol eluate to obtain 40%-60% and 70%-90% ethanol eluate respectively. The total content of 40%-60% ethanol eluate is measured by spectrophotometry....

Embodiment 3

[0055] After crushing 100g of wild jujube branches and leaves, cold immersion extraction with 10 times the amount of aqueous solution for 3 times, 3 hours each time, recover the solvent, concentrate to obtain the extract, then add 8 times the weight of the extract with distilled water, dissolve the extract under ultrasonic conditions, The above solution is passed through the D101 macroporous resin chromatography column. The amount of the macroporous resin is 10 times the weight of the extract. It is first eluted with water, washed until the phenol-sulfuric acid test solution has a negative reaction, and the flow rate is 1 column volume / hour. Gradient elution with ethanol solution with volume concentration of 10%, 30%, 40%, 60%, and 70%. The flow rate is 1 column volume / hour, and the amount is 10 times the column volume. Discard water and 10%. -30% ethanol eluent, 40%-60% and 70%-90% ethanol eluent were obtained respectively. The total flavonoid content in the 40%-60% ethanol elu...

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Abstract

The invention belongs to the technical field of medicines, relates to a wild jujube stem and leaf extract, as well as a preparation method and application thereof, and further relates to application of the wild jujube stem and leaf extract in preparation of tranquilizing, promoting sleeping and resisting depression. Chemical ingredients of the extract are mainly flavonoid ingredients and triterpene ingredients. The preparation method comprises the following steps: extracting wild jujube stems and leaves with water or ethanol solvent, and concentrating at reduced pressure to obtain extract; dispersing the extract in water, purifying with macroporous adsorption resin, mixing with 40-60% ethanol eluate and 70-90% ethanol eluate, and concentrating at reduced pressure to obtain ethanol eluate. A spectrophotometric method is used for detecting the content of total flavonoids being 45.5-60.7 percent and content of total triterpenes being 66.7-73.5 percent of each part; and the two parts are mixed to obtain an extract which has the content of total flavonoids being 15.9-21.1 percent and the content of total triterpenes being 43.3-47.8 percent. By utilizing multiple separation methods, 16 compounds are separated from the wild jujube stem and leaf extract. Pharmacological experiments prove that the wild jujube stem and leaf extract has the effects of tranquilizing, promoting sleeping and resisting depression, and can be applied to medicines for treating central nervous systems.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine chemistry, and relates to extracts of jujube branches and leaves and a preparation method and application thereof, in particular to extracts of jujube branches and leaves, a preparation method thereof and the application of the extracts in preparing sedative, hypnotic and antidepressant drugs. Background technique [0002] Epidemiological surveys show that about 35.2% of western countries have different degrees of insomnia. A statistical data from the Ministry of Health of China also shows that the number of insomniacs in my country has reached 1.2 million to 1.4 million at present, and the insomnia rate is as high as 10% to 20%. According to expert estimates, by 2020, there will be more than 700 million insomniacs worldwide. [0003] Long-term insomnia can cause a decrease in the body's immune function and metabolic disorders, and aggravate the original cardiovascular and ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P25/20A61P25/24A61P25/00
Inventor 刘晓秋潘英妮张倩倩路娟
Owner SHENYANG PHARMA UNIVERSITY
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