A method for extracting and separating diketopiperazine indole alkaloids from hemp seed
A technology for separating diketopiperazine and indole alkaloids, which is applied in the field of extracting the compound Neoechinulin A, extracting and separating diketopiperazine indole alkaloids, and can solve the problems of lack of evidence for active substances in the literature and impairment of memory and learning ability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0044] Grind the hemp seed (Cannabissativa L.) collected from Bama County, Guangxi Province, weigh 5.7Kg hemp seed, pass through an 80-mesh sieve after crushing, add 1 times the volume (g / mL) of petroleum ether, and store at 25°C Soak for 2 hours, filter under normal pressure, use 6L of ethanol with a volume fraction of 70% ethanol-water, and repeatedly percolate and extract at room temperature for 3 times, each time for 3 days, combine the extracts; concentrate to 500mL, and obtain the extract concentrate After extraction with petroleum ether, repeated extraction with 3L ethyl acetate, concentrated under reduced pressure at 30°C to a 0.25g / mL extract (measured at 50°C); Column (ODS-A-HG, 12nm, 50μm) chromatography, gradient elution with 10%, 20%, 40%, 60%, 70%, 80%, 90%, 100% methanol-water (v / v) ; 60% methanol-water (v / v) eluted concentrated extract and then MCI column chromatography, 40%, 60%, 65%, 100% methanol-water (v / v) gradient elution, using silica gel Thin-layer chr...
Embodiment 2
[0053]The hemp seed (Cannabissativa L.) collected from Bama County, Guangxi Province was crushed to obtain 4.5Kg, passed through an 80-mesh sieve after crushing, 1.5 times the volume (g / mL) of petroleum ether was added, and soaked at 30°C for 3 hours. Pressure filtration, filter residue with 4L80% ethanol-water (v / v), repeated percolation extraction at room temperature 2 times, each time for 2 days, combined extracts, concentrated to 500mL; extracted concentrate with petroleum ether, and then used 4L of ethyl acetate was repeatedly extracted, and concentrated under reduced pressure at 40°C to obtain a 0.25g / mL extract (measured at 50°C); 12nm–50nm) chromatography, with 20%, 40%, 60%, 70%, 80%, 90%, 100% methanol-water (v / v) gradient elution; 60% methanol-water (v / v) Elute the concentrated extract and then use MCI column chromatography, use 40%, 60%, 65%, 100% methanol-water (v / v) gradient elution, use silica gel thin layer chromatography to detect, wherein the developing agent...
Embodiment 3
[0055] The hemp seed (Cannabissativa L.) collected from Bama County, Guangxi Province was crushed to obtain 3Kg, passed through a 80-mesh sieve, added 2 times the volume (g / mL) of petroleum ether, soaked at 35°C for 4 hours, and filtered under normal pressure. Use 3L of 90% ethanol-water (v / v) to extract the filter residue at room temperature repeatedly for 4 times, each time for 4 days, combine the extracts and concentrate to 500mL; extract the concentrate with petroleum ether, then use 5L ethyl acetate The ester was repeatedly extracted, and concentrated under reduced pressure at 50°C to obtain an extract with a relative density of 0.25g / mL (measured at 50°C); 12nm–50nm) chromatography, with 20%, 40%, 60%, 70%, 80%, 90%, 100% methanol-water (v / v) gradient elution; 60% methanol-water (v / v) Elute the concentrated extract and then use MCI column chromatography to 40%, 60%, 65%, 100% methanol-water (v / v) gradient elution, and use silica gel thin layer chromatography to detect, w...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More