Microbial agent as well as preparation method and application thereof

A microbial agent and microbial technology, applied in the field of microbial agent and its preparation, can solve the problem of low plant seedling index

Inactive Publication Date: 2015-04-29
BEIJING JUNO SCI AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of low plant strong seedling index existing in the current vegetable seedl

Method used

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  • Microbial agent as well as preparation method and application thereof

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Example Embodiment

[0014] The present invention also provides a method for preparing the microbial inoculum, wherein the method comprises: combining Bacillus subtilis, Baclicus lincheniformis, Bacillus amyloliquefaciens, and Bacillus saffron ( Bacillus safensis) is inoculated into a medium for cultivation.

[0015] According to the present invention, the method of culture includes: culturing Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens and Bacillus saffron in separate culture systems. safensis), and mix the microorganisms obtained separately according to the ratio. Preferably, the total number of viable cells per gram of microbial inoculum is 2-10×10 by culturing in separate culture systems and mixing after culturing. 7 One.

[0016] In a preferred embodiment, by culturing in a separate culture system and mixing according to the ratio after culturing, the mixing conditions are not particularly limited, as long as the obtained microbial inoculum meets the following conditions...

Example Embodiment

[0025] Example 1

[0026] (1) Inoculate 5 parts by weight of Bacillus subtilis (ACCC01266) in 100 parts by weight of culture medium (5g glucose, 30g soybean meal, 2g peptone, 1000ml distilled water, PH7.0-7.2), cultivate at 37°C, during the cultivation process Take a sample and observe by direct counting with a microscope until the number of viable Bacillus subtilis is 2×10 7 Pieces / g of medium.

[0027] (2) Inoculate 5 parts by weight of Bacillus licheniformis (ACCC01468) in 100 parts by weight of the medium (0.45g of corn steep liquor, 1.50g of peptone, 1:15 in soy meal powder, 1.50g of glucose, pH 7.5), Cultivate at 37°C, take samples during the cultivation process and observe by direct counting with a microscope until the number of viable Bacillus licheniformis is 2×10 7 Pieces / g of medium.

[0028] (3) In 100 parts by weight of medium (50g soybean meal powder, 20g sucrose, 5g ammonium sulfate, 2.5g trisodium citrate, 0.3g potassium dihydrogen phosphate, 0.5g magnesium sulfate, ...

Example Embodiment

[0031] Example 2

[0032] (1) Inoculate 4 parts by weight of Bacillus subtilis (ACCC01266) in 100 parts by weight of medium (5g glucose, 30g soybean meal, 2g peptone, 1000ml distilled water, PH7.0-7.2), and cultivate at 37°C. Take a sample and observe by direct counting with a microscope until the number of viable Bacillus subtilis is 8×10 7 Pieces / g of medium.

[0033] (2) Inoculate 6 parts by weight of Bacillus licheniformis (ACCC01468) in 100 parts by weight of the medium (0.45g of corn steep liquor, 1.50g of peptone, 1:15 in soy meal powder, 1.50g of glucose, pH 7.5), Cultivate at 37°C, take samples during the cultivation process and observe by direct counting with a microscope until the number of viable Bacillus licheniformis is 8×10 7 Pieces / g of medium.

[0034] (3) In 100 parts by weight of medium (50g soybean meal powder, 20g sucrose, 5g ammonium sulfate, 2.5g trisodium citrate, 0.3g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.05g ferrous sulfate, PH7.0 -7.2)...

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Abstract

The invention provides a microbial agent. The microbial agent comprises a culture medium and thalli, wherein the thalli comprise Bacillus subtilis, Baclicus lincheniformis, Bacillus amyloliquefaciens and Bacillus safensis. The microbial agent provided by the invention can obtain a good plant seedling index and further improve yield and quality of vegetables, thereby having broad application prospects in the field of vegetable seedling raising. In addition, by using the microbial agent provided by the invention, a plant rhizosphere microbial environment can be improved, and beneficial bacteria are increased, thereby inhibiting the growth of harmful bacteria and further improving the disease resistance of plants.

Description

technical field [0001] The present invention relates to a microbial bacterial agent and its preparation method and application; more specifically, it relates to a microbial bacterial agent and its preparation method, and also relates to the application of the biological bacterial agent in vegetable seedling cultivation. Background technique [0002] With the development of the world's agricultural economy, the agricultural planting structure is also undergoing corresponding adjustments, gradually transforming from traditional agriculture to modern agriculture, and the planting area of ​​vegetables is also increasing year by year, and seedling cultivation is a very important technical link in vegetable cultivation , Vegetable seedlings can not only make vegetables appear on the market earlier, but also increase vegetable yield and improve quality. [0003] At present, the problems in vegetable seedling cultivation are serious soil-borne diseases in vegetable seedling cultivat...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125C12R1/10C12R1/07
CPCA01N63/00C12N1/205C12R2001/07C12R2001/10C12R2001/125A01N2300/00
Inventor 高红春朱昌雄顾金刚周艳丽
Owner BEIJING JUNO SCI AGRI TECH
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