Genetic detection kit for communicating hydrocephalus

Abstract

The invention discloses a genetic detection kit for communicating hydrocephalus. The kit comprises a RT-PCR buffer, a deoxy nucleoside triphosphate mixture, specific amplification primers and probes. The kit is convenient to sample, and venous blood samples of a to-be-detected patient can be sampled; the fluorescence amplification detection kit has the advantages of high sensitivity and strong specificity and can be used for early diagnosis; and the kit is of great significance for giving the early warning of patients with communicating hydrocephalus and taking effective treatment means.

Description

technical field [0001] The invention relates to a detection kit, in particular to a gene detection kit for communicating hydrocephalus. Background technique [0002] Hydrocephalus is divided into communicating hydrocephalus and non-communicating hydrocephalus. The formation and absorption of cerebrospinal fluid in normal people is a dynamic balance process. The formation of cerebrospinal fluid is produced by the choroid plexus of the lateral ventricle. The cerebrospinal fluid produced by the choroid plexus passes through other ventricles, flows to the subarachnoid space through the space between the cerebellum and pons, and reabsorbs through the blood vessels in the subarachnoid space to achieve balance. If there is too much cerebrospinal fluid produced by the choroid plexus or fibrosis occurs in the subarachnoid space, it will lead to communicating hydrocephalus. Children with congenital communicating hydrocephalus have no obvious clinical manifestations at first. ...

AI Technical Summary

Problems solved by technology

If there is too much cerebrospinal fluid produced by the choroid plexus or fibrosis occurs in the subarachnoid space, it will lead to communicating hydrocephalus. Children with congenital communicating hydrocephalus have no obvious clinical manifestations at first. The child showed obvious head circumference increase, language cognitive ability was affected, and limbs were paralyzed; the child needed a cerebrospinal fluid shunt operation, that is, the excessive cerebrospinal fluid was shunted from the lateral ventricle to the abdominal cavity for treatment through a catheter; therefore, the patient’s Early diagnosis is very important, which can achieve early diagnosis and early treatment; the current diagnosis is mainly based on MRI scanning technology. Through scanning the patient's brain, the Evan ratio is determined, that is, the ratio of the widest part of the lateral ventricle to the widest part of the brain. If it is greater than 0.3, it is hydrocephalus, but the cost of MRI scanning is high, it is sensitive to the patient's body movement, it is easy to produce artifacts, the scanning time is long, and some patients will have claustrophobia; and when the MRI scan is used to judge Hydrocephalus can only be diagnosed after the patient becomes ill or even seriously ill, which is not conducive to the control of the disease. If the patient with hydrocephalus is diagnosed and treated early, the disease can be effectively controlled. Low cost, high sensitivity, strong specificity, in vitro gene detection kit for early diagnosis of communicating hydrocephalus, which is of great significance for early warning and effective treatment of communicating hydrocephalus patients