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High-sensitivity and high-selectivity metal mercury ion electrochemical sensor

A high-selection, sensor technology, applied in the direction of material electrochemical variables, etc., can solve the problems of limiting detection sensitivity and selectivity, and achieve the effect of improving detection sensitivity, high detection selectivity, and good repeatability

Active Publication Date: 2015-04-29
NANJING UNIV
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Problems solved by technology

However, in these methods, each change in DNA structure requires multiple T-Hg 2+ -T bonding force to achieve, greatly limiting the detection sensitivity and selectivity of these methods

Method used

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  • High-sensitivity and high-selectivity metal mercury ion electrochemical sensor

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Embodiment 1

[0030] Embodiment 1: In conjunction with accompanying drawing 1, the detection schematic diagram of the metal mercury ion electrochemical sensor of high sensitivity and high selection

[0031] (1) Based on Au-S bonding, thiol-modified capture probes (P1) are self-assembled on the surface of the gold electrode, and then blocked by mercaptohexanol (MCH) to exclude non-specific adsorption: dropwise on the surface of a clean gold electrode 6 μL of 1 μM capture probe (P1), incubated at room temperature for 2 hours, rinsed with washing solution, dried with nitrogen, then added dropwise 6 μL of 1 mM MCH blocking solution on the surface of the electrode to block for 30 min, washed with washing solution, and dried with nitrogen.

[0032] (2) Prepare the sample incubation solution: put the 2+ The solution to be tested, the signal probe (P2) and the auxiliary probe (P3) are mixed in the Tris-HCl buffer of 10 mM pH=7.4 containing 100 mM NaCl, so that the signal probe (P2) and the auxiliar...

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Abstract

The invention relates to a high-sensitivity and high-selectivity metal mercury ion (Hg<2+>) electrochemical sensor. Based on an Au-S bonding effect, an Hg<2+>electrochemical sensor interface is constructed by self-assembling a sulfydryl-modified DNA capturing probe on the surface of a gold electrode. A synergetic hybrid effect can be generated by the strong affinity effect between thymine (T) of DNA and the Hg<2+> when the Hg<2+>, a DNA auxiliary probe and an electrochemical active molecule ferrocene (Fc) modifying DNA signal probe coexist; and a three-arm DNA Y-shaped structure is formed on the surface of the gold electrode so that an Fc molecule is close to the surface of the electrode. The size of oxidization current of the Fc is directly detected so that the concentration of the Hg<2+> in a solution is determined. The single Hg<2+> can be induced on the surface of the electrode to form one DNA Y-shaped structure so that the sensitivity and selectivity for detecting the Hg<2+> are very high. The sensor can be simply prepared, has short analysis time and good repeatability, is easy to update, and can be applied to the fields of environment monitoring, food safety and the like very well.

Description

1. Technical field [0001] The invention is a high-sensitivity and high-selection metal mercury ion electrochemical sensor. by single T-Hg 2+ -T bonding promotes a synergistic hybridization effect, forming a three-armed DNA "Y" structure on the surface of the gold electrode, and utilizing the rapid electron transfer of electrochemically active molecules on the electrode surface to achieve Hg 2+ Simple, rapid, highly sensitive, and highly selective electrochemical detection. 2. Background technology [0002] Heavy metal mercury ions (Hg 2+ ) pollution in the water body has attracted people's attention day by day. It enters the human body and accumulates in the brain, liver and other organs through the biomagnification of the food chain, causing chronic poisoning, damage to the kidneys, brain, stomach and intestinal tract, and can cause nerve damage. disorders, and even death. In addition, Hg 2+ It can combine with the amino group, dimercapto group, carboxyl group, hydroxy...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/48
Inventor 鞠熀先崔琳吴洁
Owner NANJING UNIV
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