49 results about "Glycoprotein binding" patented technology

The present invention describes derivatized polysaccharide-protein conjugates, a composition comprising one or more of such derivatized polysaccharide-protein conjugates and methods of immunizing human patients with the same. The derivatized polysaccharide-protein conjugates are purified capsular polysaccharides from Neisseria meningitidis serogroups A, C, W-135, and Y, derivatized chemically activated and selectively attached to a carrier protein by means of a covalent chemical bond, forming polysaccharide-protein conjugates capable of eliciting long-lasting immunity to a variety of N. meningitidis strains.

The invention discloses a method for reducing sialic acid loss in a bird's nest treatment process, and belongs to the field of health-care foods. The method comprises the following steps: firstly, steaming bird's nest to be treated by steam; then, soaking the steamed bird's nest by using an ice water mixture for a set time; and then, carrying out feather picking treatment on the soaked bird's nestto obtain a finished product. According to the invention, the bird's nest protein is denatured by high-temperature denaturation before feather picking, so that sialic acid combined with the protein in the bird's nest is retained. The sialic acid in the bird's nest does not exist in a monomer form and is combined with the glycoprotein in the bird's nest. When the protein is not easy to dissolve inwater after being denatured, the sialic acid in the bird's nest is not easy to dissolve in water, so that the content of the sialic acid is retained in the wet picking process. Therefore, the sialicacid in the bird's nest is completely retained in the feather picking treatment and the later product processing.

The present invention describes derivatized polysaccharide-protein conjugates, a composition comprising one or more of such derivatized polysaccharide-protein conjugates and methods of immunizing human patients with the same. The derivatized polysaccharide-protein conjugates are purified capsular polysaccharides from Neisseria meningitidis serogroups A, C, W-135, and Y, derivatized chemically activated and selectively attached to a carrier protein by means of a covalent chemical bond, forming polysaccharide-protein conjugates capable of eliciting long-lasting immunity to a variety of N. meningitidis strains.

A gene clone and expression of the oral gland KGD pattern protein of Japanese lamprey with anti-thrombosis is disclosed. Its cDNA sequence and clone, its relative protein sequence, its expression in colibacillus and Piohia yeast, its combination with the glucoprotein gpó�b/IIIa on cell membrane of platelet to suppress the platelet coagulation, and its application in developing the anti-thrombosis medicines are disclosed.

The invention relates to a prescription and a preparation method of a multi-valent pneumococcal conjugate combined vaccine. The combined vaccine is prepared by pneumococcal capsular polysaccharides selecting from pneumococcal serotypes 2, 8, 9N, 10A, 11A, 12F, 15B, 17F, 20, 22F and 33F and biologically-acceptable carrier proteins. The prescription and the preparation method provided by the invention have the benefits that a polysaccharide protein conjugate stock solution is prepared by selecting a binding method suitable for the different types of pneumococcal polysaccharides, and the different types of pneumococcal polysaccharides are combined to prepare the vaccine. The combined vaccine is used for preventing infectious diseases caused by unlisted pneumococcal polysaccharide protein-binding vaccine serotype pneumococci.

The invention discloses a method for improving immunogenicity of a 13-valent pneumococcal polysaccharide-protein conjugate. The method comprises the following steps of 1, adding ISQAVHAAHAEINEAGR OVAp into CRM197A and producing OVAp-containing CRM197A protein carrier (OVApCRM197A), and 2, connecting 13 different serotypes of polysaccharides to the OVApCRM197A by a covalent bond so that a 13-valent pneumococcal polysaccharide-OVApCRM197A conjugate is obtained. Compared with a 13-valent pneumococcal polysaccharide-CRM197A conjugate prepared from corresponding OVAp-less CRM197A, the 13-valent pneumococcal polysaccharide-OVApCRM197A conjugate improves immunogenicity by 3-5 times.

The invention discloses a preparation method of a type B haemophilus influenzae capsular polysaccharide. The preparation method comprises the steps of fermentation culture, sterilization and precipitation, polysaccharide extraction and purification, polysaccharide derivatization and polysaccharide protein conjugate preparation. The invention further discloses a multivalent combined vaccine which is prepared through the steps that an acellular pertussis stock solution, refined diphtheria toxoid and refined tetanus toxoid are added into aluminum hydroxide to be adsorbed and then added into a type B haemophilus influenzae capsular polysaccharide stock solution, and the mixed solution is diluted with normal saline. According to the preparation method, the prior art is optimized, and the high-purity low-impurity-content type B haemophilus influenzae capsular polysaccharide can be obtained; meanwhile, the type B haemophilus influenzae capsular polysaccharide is combined with multiple component vaccines to form the multivalent vaccine, the multivalent vaccine can be immune to multiple diseases simultaneously, the inoculating times are decreased, the medical risk is reduced, and the better immune effect can be obtained through one-time inoculating.

The invention relates to a method for detecting the contents of various types of specific sugar in polyvalent pneumococcal conjugate vaccine. The method comprises the following steps: step I: preparing solutions of certain concentrations from various types of monovalent capsular polysaccharide-protein conjugates according to sugar concentrations; step II: taking several parts of pneumococcal conjugate vaccine, adding the conjugate solutions obtained in the first step into the parts of pneumococcal conjugate vaccine except the first part, and diluting all the samples with a solvent to the same volume, wherein the volumes of the conjugate solutions added into the parts of pneumococcal conjugate vaccine increase from the second art to the last part in sequence; step III: adding alkali into each sample for desorption, and then adding acid for neutralization to obtain standards; step IV: carrying out reactions between the standards and a detection reagent, and drawing a standard curve by taking the sugar contents of the conjugate solutions as horizontal coordinates and taking reacting values as vertical coordinates; step V: pushing the standard curve outwards to the content axis, and taking the intercepts on the content axis as the sugar contents of monovalent capsular polysaccharide-protein conjugates in the polyvalent pneumococcal conjugate vaccine.

The invention belongs to the technical field of medicines, and discloses application of IELLQAR as medicine for preventing and treating atherosclerosis diseases. The IELLQAR can inhibit the binding ofmononuclear cells and P, E and L selection glycoprotein so as to inhibit the adhesion of mononuclear cells and endothelial cells, and also has a function of inhibiting inflammation. An ApoE gene mouse is induced by high fat diet, then obvious atherosclerosis plaques can be seen in the aorta, IELLQAR intervention can retard ApoE gene knockout mouse atherosclerosis lesions, and local mononuclear macrophage infiltration of atherosclerosis plaques can be reduced. Animal experiments and in vitro cell research levels indicate that the IELLQAR can inhibit mononuclear cell adhesion and inflammatory factor expression, atherosclerosis progress can be inhibited through an anti-inflammatory mechanism, atherosclerosis-related diseases can be prevented and treated, and therefore, the IELLQAR has an important clinical application prospect.

The invention discloses a method for determining a binding rate of a proteoglycan protein binding site in a proteoglycan protein conjugate vaccine. The method comprises the following steps of: (1) carrying out enzymolysis on a standard substance; (2) carrying out solid-phase extraction to enrich a target peptide fragment; (3) performing high performance liquid chromatography tandem mass spectrometry analysis; (4) drawing a standard curve to obtain a standard working curve equation; (5) detecting a sample, and calculating to obtain the concentration of each characteristic peptide fragment in the sample; and (6) calculating a proteoglycan binding rate of each characteristic peptide fragment and a protein residue rate of each site. According to the method, the multi-site quantitative monitoring of the proteoglycan protein binding rate of the proteoglycan protein conjugate vaccine is achieved, the tetanus protein relates to 20 monitoring sites, and the CRM197 relates to 16 monitoring sites; the method not only can detect the residual protein content, but further can evaluate the binding rate of the proteoglycan protein binding site of the proteoglycan protein conjugate vaccine, can beused for researching polysaccharide or proteoglycan protein conjugate vaccines with the same protein residual rate but different activity and toxicity, and can provide a direct monitoring method for improving a proteoglycan protein binding process.

The invention relates to a cathepsin D proenzyme ConA-pCD specifically combined with concanavalin A in serum and application thereof. The ConA-pCD is prepared by the following method: (1) preparing a concanavalin A affinity chromatography column for standby; (2) taking serum, centrifuging, taking a supernatant and adding glycoprotein bind buffer to reach a final volume of 0.5-5 ml; transferring the mixture into the prepared concanavalin A affinity chromatography column, incubating at room temperature for at least 2 h in a rotating shaking table; centrifuging, abandoning an effluent and adding a glycoprotein bind buffer containing methyl-alpha-D mannopyranoside; incubating in a rotating shaking table, centrifuging and collecting an eluate; and (3) adding acetone into the eluate to reach a concentration of 80%, staying overnight at -20 DEG C, abandoning a supernatant, drying at room temperature and dissolving by a protein lysate, so as to detect ConA-pCD. Results confirm that serum ConA-pCD is a potential and novel serum marker, with clinic application prospect, for diagnosing liver cancer.

The invention discloses a method for enhancing 13-valent pneumococcal polysaccharide protein bonder immunogenicity, and the method is as follows: adding all-powerful epitope peptide (P2) into CRM197A (A chain of diphtheria toxin variant 197), using genetic recombinant Escherichia coli to produce a P2-containing protein carrier P2CRM197A of the A chain of the diphtheria toxin variant 197 (CRM197); and connecting 13 different serotype polysaccharides with the P2-containing protein carrier P2CRM197A by covalent bonds to form a 13-valent pneumococcal polysaccharide-P2CRM197A bonder; compared with a 13-valent pneumococcal polysaccharide-CRM197A bonder obtained by a corresponding protein carrier CRM197A which does not contain the all-powerful epitope peptide (P2), the immunogenicity of the 13-valent pneumococcal polysaccharide-P2CRM197A bonder obtained by the method is increased by 3-5 times than that of a contrast.

The invention discloses an escherichia coli engineering bacterium for synthesizing glycoprotein conjugate vaccines for neonatal meningitis escherichia coli and application, and relates to a method forconstructing a cell factory for synthesizing O1 serum type glycoprotein conjugate vaccines for neonatal meningitis escherichia coli. O1 antigens are constructed based on a DNA Assembler method by utilizing efficient homologous recombination efficiency of saccharomyces cerevisiae so as to synthesize gene cluster plasmids; the O1 antigens are converted in escherichia coli JM109 to synthesize gene cluster shuttle plasmids, and the plasmids are identified by virtue of lipopolysaccharide extraction, gel electrophoresis and silver staining; waaL and wecA in the JM109 are deleted with the aid of FLP-FRT so as to eliminate the interference of original incomplete O antigens; and pET28a(+) plasmids are reconstructed and induced to synthesize the glycoprotein conjugate vaccines, the glycoprotein ispurified by virtue of an AKTA Primeplus protein purification workstation, and the glycoprotein is identified by virtue of western-blotting. The constructed recombinant escherichia coli provides a novel idea for synthesizing the glycoprotein conjugate vaccines by a biological method.

The invention relates to the technical field of electrochemistry, in particular to an electrochemical aptamer detection method of glycoprotein, which comprises the following steps: immobilizing a nucleic acid aptamer for specific recognition and capture of glycoprotein, adding a boric acid derivative of an electroactive probe after the nucleic acid aptamer is combined with target glycoprotein, quantitative analysis of the content of the target glycoprotein can be realized by measuring an oxidation-reduction signal of the electroactive probe. According to the electrochemical aptamer detection method of the glycoprotein, the electroactive probe site is labeled on the glycoprotein captured by the aptamer in a targeting manner by virtue of the affinity recognition interaction of the borate, and the electrochemical aptamer detection method can be used for high-sensitivity and high-selectivity electrochemical detection of other glycoproteins or sugar-containing substances only by replacing the sequence of the used aptamer; the method has the excellent characteristics of simplicity and convenience in operation, low cost, short detection time, high sensitivity, good selectivity and the like, and high-sensitivity electrochemical detection of glycoprotein can be realized without adopting an additional signal amplification strategy.

The present invention provides means and methods for selecting immunogenic peptides and immunogenic peptides capable of specifically binding to glycoproteins encoded by HLA alleles and inducing T cell activation in T cells restricted by the alleles combination. These peptides can be used to elicit an immune response against the desired antigen.

The present disclosure provides antibodies, and antigen-binding fragments thereof that bind to EBOV glycoprotein. The present disclosure further provides hybridoma cell lines and methods for making and using the compositions provided herein.

The invention discloses a typhoid and paratyphoid A and B polysaccharide-protein conjugated polyvalent combined vaccine. The active ingredients of the vaccine include typhoid Vi polysaccharide, paratyphoid A O-SP and paratyphoid B O-SP of salmonella as well as corresponding carrier proteins to form a conjugate. The preparation of the polyvalent combined vaccine comprises preparation combination of the polysaccharide-protein conjugate of three bacteria, dosage selection and preparation of a finished product. The results of animal experiments show that the polyvalent combined vaccine, after being injected to tested animals, can promote the tested animals to generate a high-level anti-paratyphoid A and anti-paratyphoid B lipopolysaccharide antibody (Anti-LPS IgG) and an anti-typhoid Vi polysaccharide (Anti-Vi IgG), and after boosting immunization, the vaccine has immunologic memory response and immune persistence. Meanwhile, upon vaccine safety evaluation, animal abnormal toxicity test, animal allergy test and heat source detection prove the safety and the effectiveness of the polyvalent combined vaccine.