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Cathepsin D proenzyme ConA-pCD specifically combined with concanavalin A in serum and application thereof

A cathepsin, specific binding technology, applied in the field of biotechnology applications, can solve the problems of unsatisfactory sensitivity and specificity, hindering the monitoring of high-risk groups of liver cancer, etc.

Inactive Publication Date: 2012-06-13
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the sensitivity and specificity of these new liver cancer-related markers are still not ideal. For example, the sensitivity and specificity of AFP-L3 and DCP in diagnosing liver cancer are 61-75% and 90%, respectively, but AFP-L3 is highly correlated with liver cancer. Malignancy and poor differentiation are closely related, and the sensitivity of DCP for the diagnosis of early liver cancer (< 2cm) is only 56.5%, which hinders their use in monitoring high-risk groups of liver cancer

Method used

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  • Cathepsin D proenzyme ConA-pCD specifically combined with concanavalin A in serum and application thereof
  • Cathepsin D proenzyme ConA-pCD specifically combined with concanavalin A in serum and application thereof
  • Cathepsin D proenzyme ConA-pCD specifically combined with concanavalin A in serum and application thereof

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Embodiment 1

[0037] A cathepsin D zymogen ConA-pCD that specifically binds to concanavalin A in serum is prepared by the following method: ① Preparation of concanavalin A affinity chromatography column: in a Poly-Prep chromatography column Add 100 μl concanavalin A agarose gel (purchased from Vector Laboratory), centrifuge at 500 rpm for 2 min and discard the outflow, then add 2 ml glycoprotein binding buffer containing 0.1% (v / v) Triton X-100 solution, incubated on a rotary shaker for 10 min, discarded the effluent after centrifugation, then added 2 ml glycoprotein binding buffer, incubated on a rotary shaker for 10 min, centrifuged at 500 rpm for 2 min, discarded the effluent, and set aside; the glycoprotein The binding buffer consists of: 30 mM Bis-Tris, 150mM NaCl, 1mM CaCl 2 , 1mM MnCl 2 , pH 7.2;

[0038] ②Take 20 μl of serum, centrifuge at 13,000 rpm, take 20 μl of supernatant and add glycoprotein binding buffer to a final volume of 1ml, then transfer to the above-prepared concana...

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Abstract

The invention relates to a cathepsin D proenzyme ConA-pCD specifically combined with concanavalin A in serum and application thereof. The ConA-pCD is prepared by the following method: (1) preparing a concanavalin A affinity chromatography column for standby; (2) taking serum, centrifuging, taking a supernatant and adding glycoprotein bind buffer to reach a final volume of 0.5-5 ml; transferring the mixture into the prepared concanavalin A affinity chromatography column, incubating at room temperature for at least 2 h in a rotating shaking table; centrifuging, abandoning an effluent and adding a glycoprotein bind buffer containing methyl-alpha-D mannopyranoside; incubating in a rotating shaking table, centrifuging and collecting an eluate; and (3) adding acetone into the eluate to reach a concentration of 80%, staying overnight at -20 DEG C, abandoning a supernatant, drying at room temperature and dissolving by a protein lysate, so as to detect ConA-pCD. Results confirm that serum ConA-pCD is a potential and novel serum marker, with clinic application prospect, for diagnosing liver cancer.

Description

technical field [0001] The invention belongs to the application field of biotechnology, and in particular relates to procathepsin D ConA-pCD which is isolated and purified from serum and specifically binds to concanavalin A and its application in the preparation of reagents for serological diagnosis of liver cancer. Background technique [0002] Liver cancer is one of the six most common malignant tumors in the world, with approximately 500,000 to 1 million new cases worldwide each year. Only 10-30% of newly diagnosed liver cancer patients are suitable for curative treatment (such as surgery, radiofrequency ablation and orthotopic liver transplantation). Not only that, the prognosis of liver cancer patients is extremely poor, and the 5-year survival rate is less than 5%. One of the main reasons affecting the long-term survival of patients with liver cancer is that most patients with liver cancer have developed to the middle and late stage when they are diagnosed, and the tr...

Claims

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Application Information

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IPC IPC(8): C12N9/64G01N33/574
Inventor 齐义军刘峰涛杨永杰刘瑞敏林波李涛晁玮霞张军约翰逊·菲利普马远方
Owner HENAN UNIVERSITY
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