A non-synchronous multi-column high-speed countercurrent chromatograph

Abstract

The invention discloses an asynchronous high-speed countercurrent chromatography instrument with multiple separation columns. The asynchronous high-speed countercurrent chromatography instrument comprises a rotation bracket installed in a chassis, wherein the rotation bracket is provided with at least one separation column, and one side of the chassis is provided with a complete-revolution motor for completely revolving the separation column and an autorotation motor for automatically rotating the separation column; the complete-revolution motor and the autorotation motor are independently controlled, and various different centrifugal force fields are provided by individually adjusting the autorotation and complete-revolution speeds of the separation column and the rotation direction. Different isolating environment is provided to separated samples by adjusting the autorotation and complete-revolution speeds, in comparison with a traditional high-speed countercurrent chromatography instrument, the asynchronous high-speed countercurrent chromatography instrument is unwound by a rotary sealing joint instead of a flexible hose, thus the asynchronous high-speed countercurrent chromatography instrument is characterized in that multiple gears cannot be used for unwinding, the structure is simplified, an intermediate shaft is eliminated, the beta value of the separation column is ensured, and one or more separation columns can be selectively installed.

Description

Technical field [0001] The invention relates to a high-speed countercurrent chromatograph adopting the principle of liquid-liquid distribution and separation applied in the field of biochemistry, and particularly discloses a high-speed countercurrent chromatograph with multiple separation columns that performs non-synchronous planetary rotation. Background technique [0002] The high-speed countercurrent chromatograph is a separation instrument used in the fields of biology, chemistry, and traditional Chinese medicine with liquid-liquid extraction as the separation theory. Its principle is to use the centrifugal force generated by the spiral tube during planetary motion to make the two immiscible The phase solvents are continuously mixed, and the samples are repeatedly distributed, and eluted sequentially according to the partition coefficient. Because there is no need for any solid carrier or support, it can achieve high-efficiency separation and preparation in a short time, and...

AI Technical Summary

Problems solved by technology

[0003] At present, various existing high-speed countercurrent chromatography is positioned as a general-purpose high-speed countercurrent chromatography, that is to say, different types of samples are used on the same high-speed countercurrent chromatography. Due to the difference in interfacial tension of different types of solvent systems, specific gravity and viscosity The difference makes some types of samples have better separation effect, and some types of samples have poor separation effect, which greatly reduces the use effect and efficiency of high-speed countercurrent chromatography, and cannot give full play to the advantages of high-speed countercurrent chromatography in separation and preparation.

[0004] Among them, the invention patent orthogonal axis countercurrent chromatograph (patent application number: 201310162561.7) is a high-speed countercurrent chromatograph with an orthogonal axis as the structure. The main technical problem it solves is the technology of the orthogonal axis countercurrent chromatograph. Poor balance and low separation efficiency

The revolution speed and rotation speed of the separation column are the same, only the direction of the centrifugal force field has been changed, and no technical innovation has been made for the different centrifugal force fields subjected to the revolution and rotation of the separation column

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