HPLC-HG-AFS data processing method based on theory and difference algorithms of system model

A HPLC-HG-AFS, differential algorithm technology, applied in the direction of material separation, analysis of materials, measurement devices, etc., can solve the problems affecting the accuracy of peak area reading, peak tailing, spectral peak overlap, etc. Achieve the effect of reducing calculation deviation, improving spectral resolution, and suppressing tailing

Inactive Publication Date: 2015-04-29
TIANJIN UNIV
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Problems solved by technology

Due to its own instrument structure, the HPLC-HG-AFS system will cause the tailing of the peaks. For speciation detection, if the retention times of the peaks of the various forms separated by the front HPLC system are very close, that is, the peaks are far apart. Recently, coupled with the post-column effect caused by the instrument pipeline and the peak tailing effect caused by the influence of the gas-liquid separator, the tail of the peak will be connected to the front of the rear peak, and even cause the peaks to overlap seriously. , which affects the identification of spectral peaks and the accuracy of peak area readings

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  • HPLC-HG-AFS data processing method based on theory and difference algorithms of system model
  • HPLC-HG-AFS data processing method based on theory and difference algorithms of system model
  • HPLC-HG-AFS data processing method based on theory and difference algorithms of system model

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[0020] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the implementation scope of the present invention is not limited thereto.

[0021] The present invention learns from and improves the differential algorithm of the differential GC / MS method proposed by Ghosh and Anderegg, and applies it to the analysis of spectrogram data of the HPLC-AFS system. The difference algorithm uses vector thinking, which is carried out in two steps:

[0022] The first step is to denoise and fit the original fluorescence data collected by the HPLC-HG-AFS system, and store the preprocessed data in a vector S, S=[s 1 ,s 2 ,...s n ]. Then, subtract two adjacent data points in S, and store the positive and negative difference values ​​in two vectors U and V respectively, U=[u 1 ,u 2 ,...,u n ], V=[v 1 ,v 2 ,...,v n ]. Among them, U is used to store the positive difference, and V is used to store the negative ...

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Abstract

The invention discloses an HPLC-HG-AFS data processing method based on theory and difference algorithms of a system model. The method comprises the following steps: 1 denoising and fitting original fluorescence data collected by an HPLC-HG-AFS system; and 2 executing the difference algorithm to obtain a recombined spectrogram. The differenced spectrogram is obtained after the difference algorithm is carried out once; and a primary difference algorithm is applied to the spectrogram again to obtain a secondary difference algorithm. Compared with the prior art, the fitting error can be reduced; the separation degree is improved; the linearity of a standard curve is improved; and the measurement deviation of intercept and slope of the standard curve is reduced.

Description

technical field [0001] The invention relates to an analysis and testing technology of organic forms of elements, in particular to an HPLC-HG-AFS data processing method based on system model theory and difference algorithm. Background technique [0002] When using the HPLC-HG-AFS system for element speciation analysis, the external standard method is generally used, that is, the method of concentration inversion is used to detect a series of standard substance solutions with different concentrations to obtain a standard curve, and use this standard curve to compare the samples. Measured values ​​are quantified. The accuracy of this method mainly depends on two aspects, one is the linear range of the experimental system, that is, the concentration value of the measured sample should be within the quantitative linear range of the HPLC-HG-AFS system; the other is the requirement for the standard curve The measurement must be accurate. [0003] However, there will be a lot of i...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/86
Inventor 赵学玒汪曣杜康蒋学慧孙传强孙运崔健
Owner TIANJIN UNIV
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