Novel environment-friendly nontoxic liquid-based cell preservation liquid

A liquid-based cell and preservation solution technology, which is applied in the field of new environmental-friendly liquid-based cell preservation solution, can solve the problems of medical staff safety hazards, death, environmental pollution, etc. clear effect

Inactive Publication Date: 2015-05-06
李东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the existing cell preservation solution contains methanol, isopropanol and other harmful liquids, which are poisonous and harmful, and pollute the environment. Among them, 4 milliliters of methanol can cause blindness, and 10 milliliters can cause death, which brings certain dangers to medical workers. Security risks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A new type of environment-friendly liquid-based cell preservation solution, composed of the following raw materials in parts by mass: 30% ethanol, 0.02% potassium chloride, 0.024% potassium dihydrogen phosphate, 0.144% disodium hydrogen phosphate, 0.8% sodium chloride, acetic acid Sodium 0.8%, acetic acid 0.8%, ethylenediaminetetraacetic acid 0.8%, bromhexine hydrochloride 0.03%, polyoxyethylene sorbitan monolaurate 0.8%, phenol 0.4%, and the remaining components are ultrapure water.

Embodiment 2

[0017] A new type of environment-friendly liquid-based cell preservation solution, composed of the following raw materials in parts by mass: 30% ethanol, 0.02% potassium chloride, 0.024% potassium dihydrogen phosphate, 0.144% disodium hydrogen phosphate, 0.8% sodium chloride, acetic acid Sodium 0.8%, acetic acid 0.8%, ethylenediaminetetraacetic acid 0.8%, bromhexine hydrochloride 0.02%, polyoxyethylene sorbitan monolaurate 0.8%, phenol 0.3%, and the remaining components are ultrapure water.

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PUM

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Abstract

The invention relates to liquid-based cell preservation liquid and belongs to the technical field of medicaments, and particularly relates to novel environment-friendly nontoxic liquid-based cell preservation liquid. The novel environment-friendly nontoxic liquid-based cell preservation liquid comprises ultrapure water, a pH buffer, an osmotic pressure maintaining agent, a preservative antistaling agent, a fixing agent for maintaining the cell shape, a metal chelating agent and a surfactant. The environment-friendly nontoxic liquid-based cell preservation liquid has the advantages that the main component is about 30% of ethanol, methanol, isopropanol and other toxic harmful contents are not contained, and the preservation liquid is nontoxic, harmless and environmentally friendly; the preserving rate of the cell is high, and the cell can be preserved for a long time under room temperature and the shape of the cell is good, the demarcation of the cytoplasm and the cell nucleus is clear and the gradation is distinctive, the transparency of the cytoplasm and the cell nucleus is very good, the karyoplasms is very clear and the integrity of the nucleic acid in the cell can be well preserved.

Description

technical field [0001] The invention relates to a liquid-based cell preservation solution, which belongs to the technical field of medicaments, in particular to a novel environment-friendly liquid-based cell preservation solution. Background technique [0002] At present, cervical cancer is still an important killer of women's health. Its incidence rate ranks second among female malignant tumors, second only to breast cancer. According to statistics in 2000, there are about 500,000 new cases of cervical cancer every year in the world, and there are more than 130,000 new cases in China, accounting for about a quarter of the world's new cases. About 20,000 to 30,000 people die from cervical cancer every year, and it is on the rise and younger. [0003] At present, Pap smears are commonly used for cervical cancer detection, but due to blood, mucus, inflammation and other factors in conventional Pap smears, the samples are often blurred and there are detection errors. Liquid-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 李东唐永春于惠傅琳
Owner 李东
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