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Canine interferon alpha particle compound as well as preparation method and application thereof

A technology of canine interferon and α granules, which is applied in the direction of pharmaceutical formula, medical preparations containing active ingredients, medical preparations containing active ingredients, etc., to achieve the effect of increasing curative effect, improving drug targeting, and mild preparation conditions

Active Publication Date: 2015-05-06
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, nano-medicines are mostly anti-tumor drugs and other human drugs, and there are no reports of their application in canine anti-viral drugs.

Method used

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  • Canine interferon alpha particle compound as well as preparation method and application thereof
  • Canine interferon alpha particle compound as well as preparation method and application thereof
  • Canine interferon alpha particle compound as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Synthesis of PGA-PAE material

[0031] Weigh 0.1g of γ-polyglutamic acid and dissolve it in 5ml of pure water to prepare a 2% PGA solution. Adjust the pH of the PGA solution to 3.0 with 1mol / L hydrochloric acid. Put the PGA solution in a 98°C water bath to degrade for 11 minutes, and immediately cool down in an ice bath after the degradation. Then adjust the pH to 7.0 with 1mol / L sodium hydroxide solution. 0.297g EDC.I and 0.146g phenylalanine ethyl ester were sequentially added. React on a shaker at 37°C and 210 rpm for 24 hours. After the reaction, the reaction system was centrifuged at 12000 rpm for 1 min, and the precipitate was resuspended in pure water. Repeat the centrifugation 2 times. Dry the white precipitate. Weigh, dissolve the precipitate with dimethyl sulfoxide and make up to 40mg / ml to obtain PGA-PAE material.

Embodiment 2

[0032] Example 2 Preparation of CaIFNα-NPs packaged with canine interferon alpha fusion canine interferon alpha particle complex

[0033] Take 1ml of the above-mentioned PGA-PAE material, slowly drip it into 1ml 0.25mg / ml fusion canine interferon alpha aqueous solution, and mix well to obtain a white emulsion. Centrifuge the emulsion at 16000 rpm for 15 minutes. The precipitate is separated from the supernatant. The precipitate was resuspended in 2ml of pure water and centrifuged again at 16000rpm for 15min. The precipitate is separated from the supernatant, and the precipitate is lyophilized to obtain CaIFNα-NPs.

[0034] Among them, the sequence of fused canine interferon alpha is shown in SEQ ID No:1.

[0035] In order to verify whether the fused canine interferon α is encapsulated in the particles and calculate the encapsulation rate of the fused canine interferon α, the CaIFNα-NPs were cleaved with 4% sodium dodecyl sulfonate solution. Because CaIFNα-NPs are formed by the am...

Embodiment 3

[0039] Example 3 Preparation of CaIFNα-NPs of Canine Interferon Alpha Particle Complex Coated with Free Canine Interferon Alpha

[0040] Take 1ml of the above-mentioned PGA-PAE material, slowly drop it into 1ml 0.4mg / ml free canine interferon alpha aqueous solution, and mix well to obtain a white emulsion. Centrifuge the emulsion at 16000 rpm for 15 minutes. The precipitate is separated from the supernatant. The precipitate was resuspended in 2ml of pure water and centrifuged again at 16000rpm for 15min. The precipitate is separated from the supernatant, and the precipitate is lyophilized to obtain CaIFNα-NPs.

[0041] Among them, the sequence of free canine interferon alpha is shown in SEQ ID No: 2.

[0042] In order to verify whether the free canine interferon α is encapsulated in the particles and calculate the encapsulation rate of the free canine interferon α, the CaIFNα-NPs were lysed with 4% sodium dodecyl sulfonate solution. Because CaIFNα-NPs are formed by the amphiphili...

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Abstract

The invention discloses a canine interferon alpha particle compound CaIFN alpha-NPa and a preparation method thereof. The compound comprises a gamma-polyglutamic acid-phenylalanine shell and canine interferon alpha wrapped in the shell and is formed by assembling the gamma-polyglutamic acid-phenylalanine shell and the canine interferon alpha through a self-polymerization process. The invention also discloses application of the canine interferon alpha particle compound in the preparation of canine antiviral drugs and an antiviral composition. The canine interferon alpha particle compound is high in wrapping rate, free of toxic and side effects and good in biocompatibility, can be used for retarding enzymatic degradation to realize the drug effect slow-release effect, is simple and easy to operate, low in cost and suitable for large-scale production, and has favorable application prospect.

Description

Technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to a canine interferon alpha particle complex, a preparation method and application thereof, and an antiviral composition. Background technique [0002] At present, the number of dogs raised is increasing, but the incidence of canine diseases, especially viral diseases, is constantly increasing, which often leads to the death of dogs and causes mental and economic losses to humans. Canine interferon alpha is widely used in the treatment of canine virus diseases due to its extensive antiviral activity. However, the canine interferon alpha preparations currently on the market need to be administered every day, 2-4 weeks as a course of treatment. Daily dosing and long-term cycles are not only painful for sick dogs, but also increase the mental and economic burden of the owner, and also require a lot of drugs. This situation is mainly due to the short active time of the existin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/21A61K47/34A61K9/16A61P31/12
Inventor 杨芳黄静潘盈盈章华胜严文娟谭士明陈亚州吴自荣
Owner EAST CHINA NORMAL UNIV
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