A method for rapid seedling raising of wild bamboo ginseng in sugar-free open tissue culture
A tissue culture and bamboo ginseng technology, applied in the field of plant tissue culture, can solve the problems of weak plant growth, poor leaf surface structure development, small culture container, etc., and achieve the effects of improving rooting quality, saving culture cost, and not having a fixed wavelength.
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Embodiment 1
[0034] (1), selection and disinfection of explants: take the stems of wild Panax ginseng seedlings from May to June as explants, soak them in a beaker with 75% alcohol by volume for 45 seconds to sterilize , and then washed twice with sterile water; under sterile conditions, the stems were cut into 1 cm long as explants for tissue culture;
[0035] (2) Primary sugar-free tissue culture: the explants treated in step (1) were inoculated into the primary culture medium of Panax ginseng sugar-free tissue culture, and placed in a transparent glass plant growth box with an upper opening for primary culture. The culture period is 35 days, CO 2 The concentration is 650 μL / L, the culture temperature is 24°C, and the LED light is used for illumination. The light quality red light: blue light is 2:1, the light intensity is 2000Lx, and the light time is 13 hours / day.
[0036] The sugar-free tissue culture primary culture medium of bamboo ginseng is: MS medium + 1.2mg / LGA without sugar an...
Embodiment 2
[0045] A sugar-free open tissue culture seedling raising method for wild bamboo ginseng, comprising the following steps:
[0046] (1), selection and disinfection of explants: take the leaves of wild Panax ginseng seedlings from May to June as explants, soak them in a beaker with 75% alcohol by volume for 60 seconds to sterilize , and then washed twice with sterile water; under sterile conditions, the leaves were cut into 1cm×1cm size as explants for tissue culture;
[0047] (2) Primary sugar-free tissue culture: the explants treated in step (1) were inoculated into the primary culture medium of Panax ginseng sugar-free tissue culture, and placed in a transparent glass plant growth box with an upper opening for primary culture. The culture period is 35 days, CO 2 The concentration is 650 μL / L, the culture temperature is 24°C, and the LED light is used for illumination. The light quality red light: blue light is 2:1, the light intensity is 2000Lx, and the light time is 13 hours...
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