Plant growth promoting bio-organic fertilizer for saline-alkali land
A bio-organic fertilizer and saline-alkali land technology, applied in the field of microorganisms, can solve problems such as no literature reports, and achieve the effects of being conducive to sustainable development, promoting plant growth, and preventing soil compaction
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[0031] In the following, the present invention will be further explained in conjunction with the embodiments.
[0032] (1) Production of bacterial agents
[0033] 1) Inoculate Trichoderma acnes 12 into the PDA culture solution for liquid fermentation production. The fermentation production conditions are: the initial pH range is 6.5-7.2, the culture temperature is 25-30°C, and the dissolved oxygen ventilation range is 30-100 %, 170rpm, all mycelial balls are broken into hyphal fragments in the later stage of fermentation, the colony forming unit of the fermentation broth strain is ≥1×10 8 cfu ml -1 ; The preparation method of the PDA culture medium used is (take the preparation of 1L medium as an example): Peel 100 g potatoes, cut into small pieces, put them in water and boil, boil for 30 minutes, add 5g ordinary sucrose to the filtrate after filtration And 2g rice straw powder, dilute to 1000 ml, with natural pH, sterilize at 115°C for 30 min.
[0034] 2) Inoculate the fermentation...
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