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Scenedesmus obliquus with tolerance to high pH and breeding method thereof

A technology of Scenedesmus obliquus and tolerance, which is applied in the field of microbial engineering, can solve the problems of low carbon dioxide efficiency and low solubility, and achieve the effects of improving fixation efficiency, increasing growth rate, and increasing solubility

Active Publication Date: 2015-05-13
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scenedesmus obliquus selected and bred by the present invention can better absorb and utilize carbon dioxide under high pH value, and grow and reproduce rapidly, which solves the problem that the existing Scenedesmus obliquus can only grow under neutral pH, while under neutral pH condition There are problems of low solubility of carbon dioxide and low efficiency of fixing carbon dioxide

Method used

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  • Scenedesmus obliquus with tolerance to high pH and breeding method thereof
  • Scenedesmus obliquus with tolerance to high pH and breeding method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0037] Example 1 Isolation and Screening of Mutants

[0038] The original Scenedesmus obliquus strain of the present invention is a wild Scenedesmus obliquus strain isolated from a water sample of Hunhe River in Fushun City, Liaoning Province by means of plate marking in October 2011, and the number is FSH-Y1. The original Scenedesmus obliquus strain FSH-Y1 was subjected to ultraviolet mutagenesis and domesticated culture to select a mutant strain of Scenedesmus obliquus that tolerated high pH.

[0039] Specific steps are as follows:

[0040] (1) Prepare BG11 medium, divide the prepared BG11 medium into 250mL shake flasks, each bottle has a liquid volume of 100mL, seal it with a gas-permeable film, wrap it in kraft paper, and sterilize it at 121°C and 0.1MPa for 30 minute.

[0041] (2) Under aseptic conditions, draw about 10 mL of Scenedesmus obliquus liquid FSH-Y1 and inoculate it into the above-mentioned sterilized shake flask, and place the shake flask in a constant tempe...

Embodiment 2

[0048] Comparison of the growth rate of the original algae strain and the mutant algae strain at different pH: the algae liquid in the logarithmic growth phase of the screened algae strain FSH-Y2 was inoculated with the algae liquid in the logarithmic growth phase of the original algae species in a certain proportion. into shake flasks filled with BG11 medium, the initial OD after inoculation 680 The value is the same, both are 0.2, cultivated for 15 days under different pH values, and measure the OD 680 The value is used as an index to measure the growth rate, and the results are shown in Table 3.

[0049] Table 3 Comparison of OD values ​​between original algal strains and mutagenic algal strains at different pH values

[0050]

[0051] It can be seen from Table 3 that the OD value of the mutagenized algal strains did not change significantly with the increase of pH value, while the OD value of the original algal strains decreased rapidly, grew slowly, and even died.

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Abstract

The invention provides a scenedesmus obliquus with high tolerance to high pH and a breeding method thereof. By ultraviolet mutagenesis and a step-by-step pH-increasing acclimation and screening method, scenedesmus obliquus undergoes breeding so as to obtain scenedesmus obliquus with high tolerance to high pH. The scenedesmus obliquus is named FSH-Y2 and was preserved in the China General Microbiological Culture Collection Center on September 11th, 2012, with the preservation number of CGMCC No. 6551. The selected scenedesmus obliquus can better absorb and utilize carbon dioxide at high pH value to rapidly grow and propagate. Thus, the problem that existing scenedesmus obliquus only can growth at neutral pH and carbon dioxide solubility and carbon dioxide fixation efficiency are low under the condition of neutral pH is solved.

Description

Technical field [0001] The invention is a microbial engineering field, which involves a diagonal grids and its selection methods with high tolerance. Background technique [0002] With the increasingly prominent shortage of human social resources and environmental issues, the world is facing the crisis of energy deficiency and ecological environment damage. Therefore, seeking a green renewable new energy has become a scientific issue that scientists from all over the world pay attention to.Among many creatures, microalgae have rich resources, many types, high photosynthetic utilization efficiency, fast growth rate, strong adaptability, and high synthetic oil content. In recent years, it has attracted much attention from researchers in various countries. [0003] Gallery Algae, also known as the raster algae, is a genus of the green algae gate of the green algae grille. It is a common -planted flora in fresh water.Algae (algae Scenetesmus Obliiique ), Sharp grid algae ( S. Acumina...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12N13/00C12R1/89
CPCC12N13/00C12N15/01C12N1/125C12R2001/89
Inventor 师文静高大成廖莎金平王领民李晓姝张霖王崇辉姚新武
Owner CHINA PETROLEUM & CHEM CORP
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