The invention relates to modified tussah silk fabric with functionalized nanometer chitosan and nanometer titanium dioxide. The modified tussah silk fabric is prepared by the steps in sequence: weaving the tussah silk fabric by utilizing tussah filament, preparing finishing liquor and finishing tussah silk fabric; and the technical problems that the existing textile materials in the modification technology can not effectively improve the properties that the tussah silk fabric has damage to the environment, is easy to wrinkle, not anti-bacterial and easy to shrink, has poor dyeing property, poor comfortableness and function, and the like can be solved, the comprehensive properties that the tussah silk fabric is anti-wrinkle, soft, anti-bacterial, shrink-proof, self-cleaning and ultraviolet ray-resistant, as well as the adsorption efficiency of dye, and the like can be improved and enhanced, and the tussah silk fabric has the technical benefits of being lower in preparation cost, simple in process, environment-friendly and easy to realize, etc.