Gene capable of improving quality of plant fruit as well as encoding product and application thereof
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A gene and product technology, applied in the field of improving the quality of plant fruit and its encoded product and application, can solve the problem of insufficient research on the formation and regulation mechanism of banana fruit quality
Active Publication Date: 2015-05-13
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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However, the research on the formation and regulation m
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Embodiment 1
[0034] Embodiment 1, the acquisition of transforming MaOFP1 tomato
[0035] 1. Acquisition of gene MaOFP1
[0036] Application of Clontech's MATCHMAKER TM GAL4Two-Hybrid System 3 kit, banana MuMADS1 was used as bait to construct the cDNA library of banana fruit 2 days after harvest, and the co-transformation method was used to transform yeast AH109, in SD / -Ade / -His-Leu / -Trp+x-a-gal On the screen, pick the blue colony, extract the yeast plasmid DNA as a template, and use the long-distance PCR primers in the kit (P1: 5′-CTATTCGATGATGAAGATACCCCCACCAAACCC-3′and P2: 5′-GTGAACTTGCGGGGTTTTTCAGTATCTACGATT-3′and the Advantage 2PCR Polymerase Mix (Clontech, Cat.No.639201) amplified, the obtained PCR product was sequenced, and after bioinformatics analysis, full-length primers were designed, and the amplified nucleotide sequence was SEQ ID No.1 in the sequence table, The gene shown in the nucleotide sequence is named MaOFP1, the protein encoded by it is MaOFP1, and the amino acid se...
Embodiment 2
[0053] Example 2, Functional Research of MaOFP1
[0054] 1. Phenotype of MaOFP1-transformed tomato
[0055] Sow T2 generation transformed MaOFP1 tomato (MaOFP1), T2 generation transformed empty vector tomato and untransformed wild-type tomato (control) numbered L3, L5, L6, L7, and sow the tomato seeds of the above strains in the seedling pots, at 25 ℃, light 3000lux, culture under 8h / d, and grow for about 2 months. When the plant grows vigorously and the height of the plant is 15-20cm, transfer the seedlings to the field, and observe the fruit at the red maturity stage. There were 20 strains for each strain, and the experiment was repeated three times, and the results were average ± standard deviation.
[0056] The result is as image 3 As shown, where A is the longitudinal section of red ripe fruit; B is the regulation of MaOFP1 on the weight of red ripe fruit, C and D are the regulation of MaOFP1 on the thickness of red ripe fruit peel and pulp, E is the effect of MaOFP1 o...
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Abstract
The invention discloses a novel gene MaOFP1 capable of improving the quality of a plant fruit and relates to a related application of the MaOFP1 gene in improving the quality of the plant fruit. The invention provides a method for cultivating a transgenic plant. In order to inoculate the MaOFP1 gene to a target plant to obtain a transgenic plant having at least one of the following characteristics (1)-(4): (1) the weight of the transgenic plant fruit is greater than that of the target plant fruit; (2) the thickness of the transgenic plant fruit flesh is greater than that of the target plant fruit flesh; (3) the thickness of the transgenic plant fruit peel is greater than that of the target plant fruit peel; and (4) the content of soluble solids in the transgenic plant fruit is greater than that in the target plant fruit, the nucleotide sequence of the MaOFP1 gene is SEQ ID No. 1 in sequence table or nucleotide at the 1st-435th positions from 5' terminal of the SEQ ID No. 1 in sequence table; the amino acid sequence of an encoding product MaOFP1 protein of the MaOFP1 gene is SEQ ID No. 2 in the sequence table. Experiments show that the MaOFP1 gene of a banana fruit is inoculated to a wild-type tomato to obtain the transgenic plant with improved quality and a foundation is laid for plant breeding.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a gene for improving plant fruit quality, its coding product and application. Background technique [0002] OFP (ovate family protein, OFP) (ovate family protein gene) is a class of genes that only exist in plants, and the protein encoded by this class of genes has a nuclear-like localization signal and a conserved protein of unknown function consisting of 70 amino acids. Domain of Unknown Function 623 (DUF623) domain (Liu et al., 2002), and later this domain was renamed OVATE domain (Hackbusch et al., 2005). The OVATE gene was first isolated and identified in tomato as an important regulatory factor for fruit shape. The premature appearance of the terminator during the translation process of the OVATE gene led to the transformation of tomato fruit from round to pear-shaped (Liu et al., 2002). Later, the AtOFPs gene family with a conserved OVATE domain was also found in Arabidopsis,...
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IPC IPC(8): C12N15/29C07K14/415C12N15/84A01H5/08
Inventor 金志强刘菊华徐碧玉欧阳婷李雅超张建斌贾彩红王卓苗红霞
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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