Tobacco cellulose content determination method

A technology of tobacco cellulose and measurement method, which is applied in the direction of analysis by nuclear magnetic resonance, etc., can solve the problems of unsatisfactory correlation coefficient of standard working curve, systematic error of measurement results, and failure to find, etc., to achieve accurate and reliable measurement results, and eliminate impurity peaks The effect of interference and elimination of influence

Active Publication Date: 2015-05-13
CHINA TOBACCO ANHUI IND CO LTD +1
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Problems solved by technology

However, because tobacco is a very complex chemical system, in order to eliminate the interference of other substances on cellulose, Hall and Wooten first put the tobacco samples to be tested in hot water for washing to obtain water-insoluble and water-soluble substances. , and then these two types of components are dried, and the NMR spectra of the water-insoluble and water-soluble substances are tested respectively. During data processing, the NMR spectra of the water-soluble substances are used as the background from the However, in the research process of Hall and Wooten, no suitable internal s...

Method used

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  • Tobacco cellulose content determination method
  • Tobacco cellulose content determination method
  • Tobacco cellulose content determination method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] First, accurately weigh 35.5mg, 60.6mg, 90.2mg, 119.7mg and 148.0mg of cellulose standard sample powder with a cellulose content of 90.47%, respectively, and place them in a sample tube with a silica gel tube as the internal standard. 13 cross-polarization magic angle rotating nuclear magnetic resonance spectrometer is tested to obtain the spectrograms of each sample, such as figure 1 shown.

[0031] then, yes figure 1 The cellulose peak at 105.5ppm and the internal standard peak at 0ppm in the spectrograms of five cellulose standard samples in the sample were used for baseline correction, second derivatives, Fourier deconvolution and curves using PeakFit v4.12 software Fitting processing, the quantitative peak area of ​​cellulose and the quantitative peak area of ​​the internal standard in the same spectrum were obtained.

[0032] figure 2 Among the 5 cellulose standard samples, the original curve, fitting curve and sub-peaks obtained by processing the cellulose pe...

Embodiment 2

[0037] Take by weighing 0.2g (accurately weighing to 0.0002g) tobacco sample powder to be tested (Yunyan 87X2F), parallel 5 parts (sample number 1-5), repeat the test and data processing of Example 1, obtain the average value of 5 repeated tests , standard deviation and relative standard deviation, the results are shown in Table 1.

[0038] Table 15 mean, standard deviation and relative standard deviation of repeated tests

[0039] Sample serial number

[0040] 4

Embodiment 3

[0042] Take 8 kinds of tobacco samples (sample number 1#-8#), divide each sample into two parts, one part is according to the tobacco industry standard "Tobacco and Tobacco Products Cellulose, Hemicellulose, Lignin Determination Method" method Tested, the other was tested according to the method steps described in Example 1, and the results are shown in Table 2.

[0043] Table 2 This method compares the determination results of cellulose in tobacco samples with the determination results of standard methods

[0044]

[0045] Therefore, the method for measuring tobacco cellulose content provided by the present invention is not only simple, fast, convenient, time-saving and labor-saving, but also accurate and reliable, and provides an effective technology for the tobacco industry to measure tobacco cellulose content in batches. ways and means.

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Abstract

The invention discloses a tobacco cellulose content determination method, which is characterized by comprising the following steps: firstly, weighing different mass of cellulose standard sample powder, adding the powder into sample tubes which use silicone tubes as internal standard substance, testing in a carbon 13 cross polarization magic angle spinning nuclear magnetic resonance spectrometer, and thus obtaining wave spectrogram of each cellulose standard sample, processing with FeakFit software to obtain a ratio of quantitative peak area of the standard sample in the same wave spectrogram to the quantitative peak area of the internal standard substance, by taking absolute mass of the cellulose in the standard sample as abscissa and taking the ratio as ordinate for drawing, obtaining a standard work curve; then, repeating the steps above, obtaining the ratio of the quantitative peak area of a to-be-determined sample in a to-be-determined tobacco sample wave spectrogram to the quantitative peak area of the internal standard substance, further obtaining absolute mass of the cellulose in the to-be-determined tobacco sample according to the standard work curve, thereby obtaining the cellulose content in the to-be-determined tobacco sample. The determination method needs no pretreatment, is accurate in quantification, and is applicable to determination of tobacco cellulose content in batches.

Description

technical field [0001] The invention relates to a method for measuring tobacco cellulose content, which belongs to the field of tobacco analytical chemistry research. Background technique [0002] Tobacco cellulose is an important tobacco biopolymer that mainly plays a role of mechanical support during the growth of tobacco plants. Cellulose has a direct impact on the combustion performance of tobacco leaves and the taste of cigarettes. Tobacco leaves with high cellulose content have good combustibility and enhanced fire holding power, but after burning, they endow the smoke with sharp irritation and the smell of "burning paper". Therefore, tobacco cellulose content is a valuable parameter to characterize cigarette products, and accurate quantification of cellulose content in tobacco samples is of great significance for cigarette formulation design. [0003] At present, the tobacco industry basically uses chemical methods to determine the cellulose content in tobacco, that ...

Claims

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Application Information

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IPC IPC(8): G01N24/08
Inventor 徐迎波杨俊胡永华蒋金辉田振峰陈开波葛少林王雨松田东
Owner CHINA TOBACCO ANHUI IND CO LTD
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